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60017

Sigma-Aldrich

Kaiser test kit

Synonym(s):

Ninhydrin test kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.22

Quality Level

100

General description

The Kaiser test kit is used for free amino group detection. Ninhydrin interacts with primary amines, resulting in the dark blue color of the test, indicating the completion of the coupling process.

Application

Kaiser test kit is used in:
  • Solid-phase peptide synthesis (SPPS) to detect the presence or absence of free amino groups
  • Evaluating deprotection reactions to ensure that the necessary amino groups are available for subsequent reactions and functionalization

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Target Organs

Nervous system,Kidney,Liver,Skin

Storage Class Code

3 - Flammable liquids

Flash Point(F)

62.6 °F

Flash Point(C)

17 °C

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCM2798
BCCM0367
BCCL6744
BCCL4230
BCCK9467

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Piperidine solution suitable for peptide synthesis, 20% in DMF

Sigma-Aldrich

80645

Piperidine solution

Quantitative monitoring of solid-phase peptide synthesis by the ninhydrin reaction
Sarin VK, et al.
Analytical Biochemistry, 117(1), 147-157 (1981)
John M Antos et al.
Current protocols in protein science, 89, 15-15 (2017-08-02)
Strategies for site-specific protein modification are highly desirable for the construction of conjugates containing non-genetically-encoded functional groups. Ideally, these strategies should proceed under mild conditions, and be compatible with a wide range of protein targets and non-natural moieties. The transpeptidation
Activating hidden signals by mimicking cryptic sites in a synthetic extracellular matrix
Y Zhu
Nature Communications, 14, 3635-3635 (2023)
Quantification of amino groups on halloysite surfaces using the Fmoc-method
K Fidecka
Royal Society of Chemistry Advances, 10, 13944-13948 (2020)
Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides.
E Kaiser et al.
Analytical biochemistry, 34(2), 595-598 (1970-04-01)
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