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Bathophenanthroline

for spectrophotometric det. of Fe in serum, ≥99.0%

Synonym(s):

4,7-Diphenyl-1,10-phenanthroline, BPhen

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About This Item

Empirical Formula (Hill Notation):
C24H16N2
CAS Number:
Molecular Weight:
332.40
Beilstein:
261048
EC Number:
MDL number:
UNSPSC Code:
23151817
PubChem Substance ID:
NACRES:
NA.21

Quality Level

Assay

≥99.0% (NT)
≥99.0%

form

solid

quality

for spectrophotometric det. of Fe in serum

technique(s)

UV/Vis spectroscopy: suitable

mp

218-220 °C (lit.)
218-222 °C

SMILES string

c1ccc(cc1)-c2ccnc3c2ccc4c(ccnc34)-c5ccccc5

InChI

1S/C24H16N2/c1-3-7-17(8-4-1)19-13-15-25-23-21(19)11-12-22-20(14-16-26-24(22)23)18-9-5-2-6-10-18/h1-16H

InChI key

DHDHJYNTEFLIHY-UHFFFAOYSA-N

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General description

Bathophenanthroline (BPhen) is a chelating agent and is specific for ferrous ions.

Application

Bathophenanthroline was used in an ultra-sensitive and selective nonextractive quenchofluorimetric method, in order to determine palladium (II) at μg/l Levels. It may also be used as the buffer layer to improve the performance of organic photovoltaic cells.

Other Notes

For the determination of iron in serum, in urine; for the determination of iron(II) in presence of iron(III); for the determination of total iron.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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T Mizuno
Talanta, 19(3), 369-372 (1972-03-01)
Traces of iron(II) (1-30 ppM) in the presence of iron(III) were determined (error <10%) by the bathophenanthroline method. Interference of iron(III) was eliminated by masking with sodium pyrophosphate (2.5-60 mg). The iron(II) complex was extracted with n-butanol, at pH 4.2-4.7.
L.E. Leon et al.
Analytical Chemistry, 53, 706-706 (1981)
R.D. Perry et al.
Analyst, 102, 114-114 (1977)
M.J. Seven, R.E. Peterson
Analytical Chemistry, 30, 2016-2016 (1958)
S Passarella et al.
Biochimica et biophysica acta, 1022(3), 273-282 (1990-03-01)
Incubation of intact mitochondria with aspartate aminotransferase results in efflux of malate dehydrogenase and vice versa. The export process is specific and rapid. It shows saturation kinetics with respect to the effector enzyme consistent with involvement of a receptor for

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