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11284932001

Roche

DNase I

from bovine pancreas

Synonym(s):

Dnase

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100 MG
1 820,00 kr

1 820,00 kr

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100 MG
1 820,00 kr

About This Item

Enzyme Commission number:
3.1.21.1 (BRENDA, IUBMB)
UNSPSC Code:
12352204

1 820,00 kr

Please contact Customer Service for Availability

biological source

bovine pancreas

Quality Level

100

form

lyophilized

packaging

pkg of 100 mg

manufacturer/tradename

Roche

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

General description

DNase I is a double-strand specific endonuclease that degrades DNA. Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease, which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III.[1] DNase I is one of the most well characterized endonucleases of mammalian origin.[2]

Application

DNase I has been used for tissue dissociation in conjunction with collagenases.[3][4][5]

Biochem/physiol Actions

During tissue dissociation, parts of the cells are lysed resulting in a release of DNA. Monomolecular DNA may cause clumping of cells. Addition of DNase I to the dissociation buffer leads to a degradation of this extracellular DNA, therby avoiding the loss of cells from undesired clumping.[6][7][8]

Features and Benefits

  • Lyophilized
  • Filtered through 0.2 μm pore size membrane before lyophilization
  • Glycoprotein and double-strand-specific endonuclease

Unit Definition

One unit is the enzyme activity which yields an increase in absorbance of 0.001 per minute under the assay conditions.

Preparation Note

Activator: Bivalent metal ions (Ca2+, Mg2+)
Working concentration: 0.01 to 1 mg/ml
Note: For each cell type the working concentration has to be determined individually. For optimal activity the enzyme needs 5 mM Mg2+.

Reconstitution

Reconstitute the lyophylizate in sterile double-distilled water (10 mg/ml); further dilution with PBS (phosphate buffered saline), HBSS (Hank′s Balanced Salt Solution) or medium.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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Certificates of Analysis (COA)

Lot/Batch Number
66073800
62858900
59265400
58409700
55609000

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