11112481001
Roche
Streptavidin-β-Gal conjugate
lyophilized (clear, colorless solution after reconstitution), pkg of 500 U
Synonym(s):
streptavidin
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About This Item
UNSPSC Code:
12352200
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form
lyophilized (clear, colorless solution after reconstitution)
packaging
pkg of 500 U
manufacturer/tradename
Roche
storage temp.
−20°C
General description
Streptavidin is covalently coupled to β-galactosidase from E. coli.
Application
- Use Streptavidin-β-Gal to detect biotinylated compounds using: ELISA
- Immunohistocytochemistry
- Western blot
- In situ hybidization
Preparation Note
Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
Storage conditions (working solution): The reconstituted solution is stable at 2 to 8 °C for four weeks. Aliquots should be stored at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.
- ELISA: 100 to 500 mU/ml
- Immunohistocytochemistry: 250 to 500 mU/ml
- Western blot: 250 to 500 mU/ml
Storage conditions (working solution): The reconstituted solution is stable at 2 to 8 °C for four weeks. Aliquots should be stored at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.
Reconstitution
Add 1 ml double-distilled water to a final concentration of 500 U/ml.
Other Notes
For life science research only. Not for use in diagnostic procedures.
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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