The test method used complies with USP 85 and EP 2.6.14 to ensure the absence of endotoxin in our products.
S2VPT10RE
Millipore® Steritop® Vacuum Bottle Top Filter
pore size 0.1 μm, polyethersulfone membrane, funnel capacity 1000 mL, pack of 12 ea
Synonym(s):
SCVPT10RE, Steritop Quick Release-VP Sterile Vacuum Filtration System, bottle top sterile filter unit, bottle top vacuum filter, bottle top vacuum filtering unit
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About This Item
Recommended Products
material
polyethersulfone membrane
polystyrene bottle
polystyrene funnel(s)
polystyrene housing
Quality Level
sterility
sterile; irradiated
product line
Steritop®
feature
hydrophilic
packaging
pack of 12 ea
parameter
max. temp. at 45 °C
description
2.4 mL (hold-up volume)
technique(s)
sterile filtration: suitable
H
169 mm
capacity
1000 mL
diam.
13.5 cm
filter diam.
73 mm
filtration area
40 cm2
funnel capacity
1000 mL
matrix
Express PLUS™
pore size
0.1 μm pore size
fitting
inlet funnel
45 mm outlet thread(s)
shipped in
ambient
General description
This Steritop ® 1000 mL bottle-top with 0.1 μm pore size polyethersulfone (PES) membrane filter unit can be used on bottles with 45 mm openings, designed for vacuum applications.
Application
- Tissue culture media +/- additives
- Buffers
- Biological solutions
Features and Benefits
Legal Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Evaluation of mycoplasma removal by sterilizing filtration using 0.1 µm PES and PVDF filters for cell culture media and buffer preparation.
Detect mycoplasma contamination in cell culture through the PCR, DNA stain, or culture tests. Discover mycoplasma prevention, elimination, and detection kits.
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What Bacterial Endotoxins Test is used?
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What is the maximum vacuum pressure for filtration?
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700 mm Hg (27.5” Hg, 0.9 bar) differential vacuum at 25 °C (77 °F)
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What tubing can be used with Stericup® and Steritop® vacuum filters?
What tubing can be used with Stericup® and Steritop® vacuum filters?
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Stericup® and Steritop® filters are fitted with a 1/4” tube adapter. We recommend 3/16 in. (4.8 mm) ID silicone tubing to fit this tube adapter: https://www.sigmaaldrich.com/product/mm/xx7100004
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What is the difference between classic Stericup® filters and Stericup® “Quick Release” filters?
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Stericup® Quick Release, or “QR”, filters feature a connection between funnel filter and receiver bottle that requires only a quarter-turn release to facilitate funnel removal. These filters come fully engaged – there is no need to tighten before use. The receiver bottle cap has flanges on the top surface to improve grip, as well as a positive lock stop to ensure a leak-proof seal with the receiver bottle. Other improvements include a large, frosted writing surface on the receiver bottle and a lighter cap color to facilitate labelling, more prominent volume markings on the filtration funnel and receiver bottle, and a product label with a peel-off lot record strip on the bag for easy viewing in storage.
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Can Stericup® and Steritop® filters be used to prepare solutions that will be administered to patients?
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No, all Stericup® and Steritop® devices are Research Use Only.
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What is the expiration date of Stericup® and Steritop® filters?
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Typically 3 years from sterilization.
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Can Stericup® bottles be placed inside freezers for long-term storage of media?
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You can successfully freeze and store many aqueous solutions (such as culture media) in Stericup® bottles at temperatures up to -20 °C (-4 °F). It is strongly recommended that you run a sample stability trial under your actual storage conditions prior to using Stericup® bottles for frozen storage.
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What is the maximum processing volume for Stericup® and Steritop® filters? Can I add more volume after initial filtration?
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All Stericup® and Steritop® filters were developed for single-use application. The maximum volume is dependent on the receiver bottle capacity. Stericup® filters come with a receiver bottle with a specified maximum volume. For Steritop® filters, the maximum recommended volume is 2 L with an appropriate-sized receiver bottle. Larger processing volumes can result in membrane fouling, increase chance of spillage and contamination, and lead to possible airlock*.
*Note that devices with PES membranes can airlock if filtered to dryness. Some liquid must be left on top of the filter, then the system stopped, receiver changed or emptied, and more sample volume added before restart. This increases the chance of spillage.Helpful?
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Can we filter oil-type liquids with Stericup® filters?
Can we filter oil-type liquids with Stericup® filters?
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Likely not, due to pore size/possible fouling/chemical compatibility. Refer to our guide, "Chemical compatibility of filter components": https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/302/855/membrane-chemical-compatibility-ds2532en-ms.pdf
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What leachables are detected during filtration processes using Stericup® filters?
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We have not performed extractables/leachables studies for our Stericup® filters.
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