MKI2MAG-94K

MILLIPLEX^® Mouse Kidney Injury Magnetic Bead Panel 2 - Toxicity Multiplex Assay

The analytes available for this multiplex kit are: Clusterin, Cystatin C, EGF, Lipocalin-2/NGAL, Osteopontin (OPN) (for urine samples) or Clusterin, Cystatin C, Lipocalin-2/NGAL, Osteopontin (OPN) (for serum/plasma samples).

• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

Properties

• Quality Level: 200
• species reactivity: mouse
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 90-99%; sensitivity: 0.005-0.190 ng/mL; (MinDC+2SD); standard curve range: 0.005-5 ng/mL; (Lipocalin-2/NGAL); standard curve range: 0.01-10 ng/mL; (Osteopontin (OPN)); standard curve range: 0.04-40 ng/mL; (EGF); standard curve range: 0.05-50 ng/mL; (Cystatin C); standard curve range: 0.2-250 ng/mL; (Clusterin)
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

Maintenance of physiological balance in the body is attributed in part to normal kidney function. An altered kidney function due to injury, drug toxicity or kidney failure may be life threatening. There are multiple risk factors for kidney injury including age, hypertension, diabetes mellitus, heart failure and liver failure. On the other hand, drug induced damage to the kidney results from drug excretion. Traditional markers for assessing renal toxicity, such as blood urea nitrogen (BUN) and serum creatinine, are insensitive and non-specific. Although both are direct measurements of renal function, increases in serum concentrations of these biomarkers occur only after substantial renal injury. Specificity and earlier detection of kidney injury is vital. For improved detection of acute nephrotoxicity, a panel of novel urinary kidney biomarkers has been identified and validated for preclinical studies in relevant animal species. We provide valuable research assays to investigate multiple biomarkers of kidney injury in mouse urine samples using the Luminex^® xMAP^® instrument platform.

The MILLIPLEX^® Mouse Kidney Injury Bead Panel 2 contains all the components necessary to measure the following five biomarkers in any combination using Luminex^® xMAP^® technology: Clusterin, Cystatin C, EGF, Lipocalin-2/NGAL, Osteopontin (OPN). The kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls and can measure up to 38 urine samples in duplicate.

Panel Type: Toxicity

Specificity

There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

Application

• Analytes: Clusterin, Cystatin C, EGF, Lipocalin-2/NGAL, Osteopontin (OPN)
• Recommended Sample type: urine
• Recommended Sample dilution: 1:1,000
• Assay Run Time: overnight
• Research Category: Toxicity

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

Everything you need in a single kit.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Please contact Technical Service for linearity of dilution.

Sensitivity: Please see kit protocol for individual assay sensitivities.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS06,GHS08,GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H332,H311,H317,H318,H373,H411
• Precautionary Statements: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• Target Organs: Respiratory Tract
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects