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IM1012

Sigma-Aldrich

Anti-MTA3 (400-450) Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-Metastasis-associated gene 3

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... MTA3(57504)

General description

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~62 kDa MTA3 protein.
Recognizes the ~62 kDa MTA3 protein in MCF-7 cells.
This Anti-MTA3 (400-450) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of MTA3 (400-450).

Immunogen

Human
a synthetic peptide corresponding to amino acids within residues 400-450 of human MTA3

Application



Immunoblotting (1:500-1:5000)
Immunocytochemistry (1:300)
Immunoprecipitation (2-10 g/mg lysate)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Highly Toxic (H)

Physical form

In Tris-citrate/phosphate buffer, pH 7.0-8.0.

Reconstitution

Following initial use, aliquot and freeze (-20°C) for long-term storage.

Analysis Note

Positive Control
MCF7 cells

Other Notes

Antibody should be titrated for optimal results in individual systems.
Fujita, N., et. al. 2003. Cell113, 207.
Kumar R. 2003. Cell113, 142.
Simpson, A, et.al. 2001. Gene273, 29.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The metastasis-associated gene MTA3 is an independent prognostic parameter in uterine non-endometrioid carcinomas.
Ioannis Mylonas et al.
Histopathology, 60(4), 665-670 (2012-01-13)
B Belinda Ding et al.
Journal of immunology (Baltimore, Md. : 1950), 190(4), 1827-1836 (2013-01-18)
After undergoing Ig somatic hypermutation and Ag selection, germinal center (GC) B cells terminally differentiate into either memory or plasma cells (PCs). It is known that the CD40L and IL-21/STAT3 signaling pathways play critical roles in this process, yet it
Jie Gao et al.
Cell death and differentiation, 28(9), 2818-2836 (2021-05-07)
The biological function of PRMT5 remains poorly understood in cervical cancer metastasis. Here, we report that PRMT5 physically associates with the transcription factor Snail and the NuRD(MTA1) complex to form a transcriptional-repressive complex that catalyzes the symmetrical histone dimethylation and
Xin Yin et al.
Cell death and differentiation, 29(11), 2203-2217 (2022-05-10)
Runt-related transcription factor 2 (RUNX2) is an osteogenesis-related transcription factor that has emerged as a prominent transcription repressing factor in carcinogenesis. However, the role of RUNX2 in breast cancer metastasis remains poorly understood. Here, we show that RUNX2 recruits the
Ruiqiong Liu et al.
Nucleic acids research, 46(13), 6608-6626 (2018-05-31)
Histone post-translational modifications regulate chromatin structure and function largely through interactions with effector proteins that often contain multiple histone-binding domains. PHF1 [plant homeodomain (PHD) finger protein 1], which contains two kinds of histone reader modules, a Tudor domain and two

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