Activator Reagent 0.3M 5-benzylmercaptotetrazole (BMT) in acetonitrile (AcN) is also known as benzylthiotetrazole (BTT) or benzyl-1H-tetrazole). It is used as a phosphoramidite activator reagent for oligonucleotide synthesis.
Application
0.3M BMT in AcN can be used as a reagent in solid phase phosphorylation of the oligomers. The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the 5′hydroxyl group of the growing oligonucleotide chain. The high activation potential of this reagent may be due to its greater acidity (pKa=4.08), which makes it easier to protonate the trivalent phosphorus in the initial stage of activation and increase the speed of the coupling reaction.
Features and Benefits
efficient and rapid activation reagent that produces less
phosphoramidite waste
higher solubility at lower temperatures
does not crystallize or clog lines
safer reagent to use
Analysis Note
Color (APHA): 10APHA max Component: Acetonitrile Purity (By GC): 99.9% min Form: Clear Liquid Identity (IR-spectrum): Conforms Molarity of 5-Benzylmercaptotetrazole: 0.28-0.32M Water (H2O): 1-30ppm
Legal Information
Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany
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