AP132B
Goat Anti-Rabbit IgG Antibody, biotin-SP conjugate
Chemicon®, from goat
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46
Recommended Products
biological source
goat
Quality Level
conjugate
biotin conjugate
antibody form
affinity purified immunoglobulin
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
rabbit
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
shipped in
wet ice
target post-translational modification
unmodified
General description
Affinity purified goat anti-rabbit IgG, Biotin conjugated.
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Application
EIA: 1:50,000-1:100,000
Western blots using enzyme-conjugated streptavidin: 1:10,000-1:100,000.
Immunohistochemistry: 1:500-1:5,000
Flow cytometry: 1:200-1:1,000
Fluorescence Immunohisto/cytochemistry: 1:200-1:1,000
Optimal working dilutions must be determined by end user.
Western blots using enzyme-conjugated streptavidin: 1:10,000-1:100,000.
Immunohistochemistry: 1:500-1:5,000
Flow cytometry: 1:200-1:1,000
Fluorescence Immunohisto/cytochemistry: 1:200-1:1,000
Optimal working dilutions must be determined by end user.
Goat anti-Rabbit IgG Antibody, biotin-SP conjugate detects level of Rabbit IgG & has been published & validated for use in ELISA & WB.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Whole Immunoglobulin Secondary Antibodies
Physical form
ImmunoAffinity Purified
RECONSTITUTION:
Reconstitute with sterile distilled water to match the volume indicated on the vial label.
Reconstitute with sterile distilled water to match the volume indicated on the vial label.
Storage and Stability
Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING:
For research use only; not for use as a diagnostic.
WARNING:
For research use only; not for use as a diagnostic.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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