AB9094-I
Anti-Sodium Pump Subunit alpha-2 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Sodium/potassium-transporting ATPase subunit alpha-2, Na(+)/K(+) ATPase alpha-2 subunit, Na(+)/K(+) ATPase alpha(+) subunit, Sodium pump subunit alpha-2, Sodium Pump Subunit alpha-2, Anti-Sodium Pump (Na+ pump (Na+-K+-ATPase)) α 2 Isoform, Sodium/po
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat, human
species reactivity (predicted by homology)
porcine (based on 100% sequence homology), bovine (based on 100% sequence homology)
technique(s)
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ATP1A2(477)
rat ... Atp1A2(24212)
General description
Sodium/potassium-transporting ATPase subunit alpha-2 (EC 3.6.3.9; UniProt P06686; also known as Na+/K+ -ATPase alpha 2 subunit, Na(+)/K(+) ATPase alpha(+) subunit, Sodium pump subunit alpha-2) is encoded by the Atp1a2 (also known as RATATPA2) gene (Gene ID 24212) in rat. Mammalian Na+/K+ ATPase is a heteromultimeric complex composed of alpha, beta, and gamma subunits. The alpha subunit catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ across the plasma membrane. The alpha subunit contains ten transmembrane domains with both its N- and C-termini in the cytoplasm. More than 50 mutations have been identified affecting the human ATP1A2 and ATP1A3 genes that are known to cause rapid-onset Dystonia Parkinsonism, familial hemiplegic migraine, alternating hemiplegia of childhood, and variants of familial hemiplegic migraine with neurological complications including seizures and various mood disorders.
Specificity
Reacts with Sodium Pump (Na+ pump (Na+-K+-ATPase)) alpha 2 isoform. The antibody does not cross react with other Na+-K+-ATPase alpha isoforms.
Immunogen
Epitope: Second cytoplasmic domain
KLH-conjugated linear peptide corresponding to the second cytoplasmic domain of rat Sodium Pump Subunit alpha-2.
Application
Detect Sodium Pump Subunit alpha-2 using this Anti-Sodium Pump Subunit alpha-2 Antibody validated for use in Western Blotting, Immunohistochemistry (Paraffin) and Immunoprecipitation.
Immunohistochemistry Analysis (IHC): A representative lot detected Sodium Pump Subunit alpha-2 in human (1:1,000) and rat (1:250) cerebellum tissue sections.
Immunoprecipitation Analysis (IP): 10 ug from a representative lot immunoprecipitated Sodium Pump Subunit alpha-2 in 500 µg of rat brain tissue lysate.
Immunoprecipitation Analysis (IP): 10 ug from a representative lot immunoprecipitated Sodium Pump Subunit alpha-2 in 500 µg of rat brain tissue lysate.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Signaling
Developmental Signaling
Quality
Evaluated by Western Blotting in rat brain tissue lysate.
Western Blotting Analysis: A 1:4,000 dilution of this antibody detected Sodium Pump Subunit alpha-2 in 10 µg of rat brain tissue lysate.
Western Blotting Analysis: A 1:4,000 dilution of this antibody detected Sodium Pump Subunit alpha-2 in 10 µg of rat brain tissue lysate.
Target description
~100 kDa observed
Linkage
Replaces: AB9094
Physical form
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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