324796

Endopeptidase Lys-C, Achromobacter lyticus

• Synonym(s): Endopeptidase Lys-C, Achromobacter lyticus
• CAS Number: 78642-25-8
• UNSPSC Code: 12352202

Properties

• form: lyophilized
• Quality Level: 100
• specific activity: ≥2 AU/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• technique(s): mass spectrometry (MS): suitable
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Native endopeptidase Lys-C from Achromobacter lyticus. Lysyl endopeptidase is a serine protease secreted by soil bacteria and specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues.

Native endopeptidase Lys-C from Achromobacter lyticus. Lysyl endopeptidase is a serine protease secreted by soil bacteria and specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues. Optimal pH: 9.0-9.5.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One AU (amidase unit) is defined as the amount of enzyme that catalyzes the release of 1 µM pNA from N-benzoyl-DL-lysine-pNA per min at 30°C, pH 9.5.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 year at -20°C.

Reconstitute 1 vial with 1 ml solvent; if the enzyme is not completely solubilized, add 1-3 ml additional solvent until completely dissolved.

Other Notes

Jekel, P.A., et al. 1983. Anal. Biochem. 134, 347.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany