230914

Chymotrypsin Substrate II, Fluorogenic

Sensitive fluorogenic substrate for the quantitative determination of chymotrypsin activity.

• Synonym(s): Chymotrypsin Substrate II, Fluorogenic, Suc-AAPF-AMC
• Empirical Formula (Hill Notation): C₃₄H₃₉N₅O₉
• CAS Number: 88467-45-2
• Molecular Weight: 661.70
• MDL number: MFCD00080246
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥98% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated; protect from light
• color: white
• solubility: DMSO: soluble; acetonitrile: water (1:1): soluble
• fluorescence: λ_ex ~380 nm; λ_em ~460 nm
• shipped in: ambient
• storage temp.: −20°C
• InChI: 1S/C34H39N5O9/c1-19-16-30(43)48-27-18-23(11-12-24(19)27)37-25(17-22-8-5-4-6-9-22)32(45)38-33(46)26-10-7-15-39(26)34(47)21(3)36-31(44)20(2)35-28(40)13-14-29(41)42/h4-6,8-9,11-12,16,18,20-21,25-26,37H,7,10,13-15,17H2,1-3H3,(H,35,40)(H,36,44)(H,41,42)(H,38,45,46)/t20-,21-,25-,26-/m0/s1
• InChI key: IGKPQFYMWKQDQS-KCXKOMAXSA-N

Description

General description

Sensitive fluorogenic substrate for the quantitative determination of chymotrypsin activity (excitation maximum: ~380 nm; emission maximum: ~460 nm).

Sensitive fluorogenic substrate for the quantitative determination of chymotrypsin activity.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Chymotrypsin

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

Suc-Ala-Ala-Pro-Phe-AMC

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Irvine, G.B., et al. 1990. Anal. Biochem.185, 304.
Graf, L., et al. 1987. Biochemistry26, 2616.
Oshima, G. 1984. J. Biochem.95, 1131.
Oshima, G. 1983. J. Biochem.94, 1615.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable