STR1
Enhanced Avian First Strand Synthesis Kit
Components for cDNA synthesis with enhanced AMV reverse transcriptase
Synonym(s):
Reverse Transcriptase cDNA synthesis kit, eAMV-RT
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About This Item
NACRES:
NA.55
UNSPSC Code:
12352200
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usage
sufficient for 50 reactions, kit sufficient for 50 reactions
feature
dNTPs included, hotstart: no
technique(s)
RT-PCR: suitable, cDNA synthesis: suitable
Quality Level
color
colorless
input
purified RNA
shipped in
dry ice
storage temp.
−20°C
Related Categories
1 of 4
This Item | |||
|---|---|---|---|
| usage sufficient for 50 reactions, kit sufficient for 50 reactions | usage sufficient for 50 reactions | usage sufficient for 100 reactions | usage sufficient for 50 reactions |
| technique(s) RT-PCR: suitable | technique(s) RT-PCR: suitable | technique(s) RT-PCR: suitable | technique(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 20-25°C |
| shipped in dry ice | shipped in wet ice | shipped in wet ice | shipped in - |
| input purified RNA | input purified RNA | input purified RNA | input - |
![Enhanced Avian Reverse Transcriptase [eAMV™ RT] For reverse transcription at higher temperatures & rare mRNAs](/deepweb/assets/sigmaaldrich/product/images/496/245/af9bcef6-1474-494b-8fba-32ca0fd56c42/640/af9bcef6-1474-494b-8fba-32ca0fd56c42.jpg)
General description
Enhanced Avian First Strand Synthesis Kit utilizes a highly purified avian myeloblastosis virus reverse transcriptase (eAMV-RT) that offers superior performance in comparison to standard AMV-RT or standard Moloney murine leukemia virus reverse transcriptase (MMLV-RT). This exceptionally robust eAMV-RT has an enhanced ability to transcribe through difficult secondary structure at elevated temperatures (up to 65 °C) making it the ideal enzyme for producing high quality full-length cDNA from total RNA or poly(A)+ RNA.
Sigma′s Enhanced Avian RT First Strand Synthesis Kit also provides random nonamers (9-mers) and anchored oligo (dT)23 primers since gene-specific primers may not always be useful or possible. The anchored oligo has 23 thymidine residues and one G, C, or A residue (the anchor). The anchor ensures that the oligo (dT) primer binds at the very beginning of the message and that there is not a long region of the unusable sequence. With this kit, a dependable cDNA is generated that can be used for various downstream applications, including PCR.
Application
Other Notes
One unit incorporates one nanomole of TMP into TCA-precipitable material in 10 minutes using polyadenylic acid as template and oligo(dT)12-18 as primer.
Legal Information
Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
Kit Components Only
Product No.
Description
- Enhanced Avian Reverse Transcriptase 1000 U
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
signalword
Danger
hcodes
Hazard Classifications
ED ENV 1
wgk
WGK 3
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