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SAB4200179

Sigma-Aldrich

Anti-Importin 5 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-IPO5

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~123 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 2.5-5.0 μg using lysates of human HEK-293 cells.
indirect immunofluorescence: 2.5-5.0 μg/mL using HEK-293 cells overexpressing human Importin 5.
western blot: 2-4 μg/mL using whole extracts of human Jurkat cells.

UniProt accession no.

O00410

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... IPO5(3843)
mouse ... Ipo5(70572)
rat ... Ipo5(306182)

General description

In human cells, there are more than 20 members of the importins/exportins family. Importin-5 (IPO5) belongs to the importin-β superfamily and is mapped to human chromosome 13q32.2.

Application

Anti-Importin 5 (C-terminal) antibody produced in rabbit has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence

Biochem/physiol Actions

Nucleocytoplasmic transport of proteins and RNAs is mediated through nuclear pore complexes (NPCs) and by karyopherins nuclear transport receptors (importins/exportins). Importins mediate either the nuclear import of a very diverse set of protein or RNA cargoes. The function of importins are regulated by the Ran G protein. Ran G protein stimulates the release of cargo from importins. Importins are implicated in anti-aggregation activity of highly basic cargo like histones and ribosomal proteins and in preventing their undesired interactions with other cellular components.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The full-length interleukin-33 (FLIL33)-importin-5 interaction does not regulate nuclear localization of FLIL33 but controls its intracellular degradation
Clerman A, et al.
The Journal of biological chemistry, 292(52), 21653-21661 (2017)
Genome-wide association study of peripheral arterial disease in a Japanese population
Matsukura M, et al.
Testing, 10(10), e0139262-e0139262 (2015)
Pauline J van der Watt et al.
International journal of cancer, 150(2), 347-361 (2021-10-01)
Previous studies have identified increased expression of members of the nuclear transport protein family in cancer cells. Recently, certain nuclear transport proteins have been reported to be secreted by cells and found in the serum. The aims of our study
Structural biology of nucleocytoplasmic transport
Cook A, et al.
Annual Review of Biochemistry, 76(3), 647-671 (2007)
Importins fulfil a dual function as nuclear import receptors and cytoplasmic chaperones for exposed basic domains
Jakel S, et al.
The Embo Journal, 21(3), 377-386 (2002)

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