indirect immunofluorescence: 1:2,000 using 3% paraformaldehyde-fixed, 0.5% Triton X-100 treated human epidermal carcinoma A431cell line microarray: suitable western blot: 1:2,000 using whole cell extract of the human epidermal carcinoma A431 cell line
Anti-SGK is developed in rabbit using a synthetic peptide corresponding to the C-terminus of human SGK conjugated to KLH (keyhole limpet hemocyanin). SGK is localized to the perinuclear or cytoplasmic compartment as a hypophosphorylated protein.
SGK (serum and glucocorticoid-regulated protein kinase) is a 50 kDa member of Ser/Thr kinase family. Sequence of sgk is well conserved across species, the human and rat SGK amino acid sequences are 96-98% identical.
Application
Anti-SGK antibody produced in rabbit has been used in :
immunoprecipitation
western blotting
immunofluorescence
Biochem/physiol Actions
SGK (serum and glucocorticoid-regulated protein kinase) plays an important role in signal transduction activated by mineral corticoids. It is also regulated at the transcription level by stimuli including glucocorticoid, serum, follicle stimulating hormone as well as changes in cell volume. SGK levels are strongly affected by osmotic changes. SGK is strongly and rapidly stimulated in kidney cells in response to aldosterone and stimulates epithelial Na+ channels, suggesting a central role of SGK in the regulation of sodium transport and homeostasis. SGK transcription is markedly increased in diabetic nephropathy, in response to excessive extracellular glucose concentrations. The localization of SGK in the cell is regulated by hormones or serum. For cell cycle progression, the shuttling of SGK between nucleus and cytoplasm is required.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Seawater acclimation in killifish, Fundulus heteroclitus, is mediated in part by a rapid (1h) translocation of CFTR Cl(-) channels from an intracellular pool to the plasma membrane in gill and increased CFTR-mediated Cl(-) secretion. This effect is mediated by serum
cell cycle and hormonal control of nuclear-cytoplasmic localization of the serum-and glucocorticoid-inducible protein kinase, sgk, in mammary tumor cells a novel convergence point of anti-proliferative and proliferative cell signaling pathways
Buse P, et al.
The Journal of biological chemistry, 274(11), 7253-7263 (1999)
Sgk is an aldosterone-induced kinase in the renal collecting duct Effects on epithelial Na+ channels
Naray FTA, et al.
The Journal of Biological Chemistry, 274(24), 16973-16978 (1999)
K v7 channels in the nucleus accumbens are altered by chronic drinking and are targets for reducing alcohol consumption
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