Elemental analysis of this material has not been performed. The sulfate content is not determined.
P8169
Phytagel™
BioReagent, suitable for plant cell culture, powder
Phytagel™
Synonym(s):
Gellan Gum, Agar substitute gelling agent
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About This Item
Pricing and availability is not currently available.
product line
BioReagent
Quality Segment
form
powder
technique(s)
cell culture | plant: suitable
application(s)
agriculture
General description
Typical working concentration: 1.5-2.5 g/L in plant tissue culture media; up to 10 g/L in microbiological media. Phytagel™ requires the presence of cations (especially divalent) for gelling to occur. Concentrations of calcium and magnesium contained in most plant tissue culture media are typically sufficient for gelation. Low-salt media formulations, especially those used in microbiological applications, may require supplementation with additional calcium or magnesium salts (e.g., CaCl2 or MgSO4) or higher concentrations of Phytagel.
Application
Phytagel™ has been used:
- in the solidification of culture medium for root assays in Arabidopsis thaliana
- as a component of Solid Paul′s medium for Agrobacterium culture
- as a component of root induction medium in Ilex dumosa tissue culture
Biochem/physiol Actions
Phytagel is intended for use in place of agar and other gelling agent for plant tissue culture. Phytagel has been shown to be a superior substitute for tissue-culture-grade agar in the micropropagation of banana plantlets.
Preparation Note
To prevent clumping, add slowly to rapidly stirring medium prior to heating.
Other Notes
An agar substitute produced from a bacterial fermentation composed of glucuronic acid, rhamnose and glucose. It produces a clear, colorless, high strength gel which aids in detection of microbial contamination.
Legal Information
Phytagel is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
|---|---|---|---|
| application(s) agriculture | application(s) agriculture | application(s) agriculture | application(s) agriculture |
| form powder | form - | form powder | form powder |
| technique(s) cell culture | plant: suitable | technique(s) - | technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| product line BioReagent | product line - | product line - | product line - |
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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What is the sulfate content, if any, of Phytagel?
1 answer-
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Buongiorno. Vorrei sapere se il Phytagel è Gellano ad alto o a basso grado di acilazione
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This product is characterized as having a low acyl content.
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What is the molecular weight of one repeating unit?
1 answer-
The molecular weight is not determined. However, the constituent sugars of Phytagel are glucose, glucuronic acid and rhamnose in the molar ratio of 2:1:1.
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What is the gelling strength of Phytagel P8169
1 answer-
The Gel Strength for Phytagel (P8169) is 700 g/cm2 (tested at 0.5%).
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