P7828

Percoll^®

suitable for plant cell culture

• Synonym(s): density gradient medium
• UNSPSC Code: 10171502
• NACRES: NA.72
• MDL number: MFCD00131830

Properties

• sterility: aseptically filled
• Quality Segment: 200
• form: colloidal (Colloidal solution of silica coated with polyvinylpyrrolidone (PVP))
• technique(s): cell culture | plant: suitable
• pH: 8.9 (20 °C)
• application(s): agriculture
• storage temp.: 2-8°C

Description

Application

Percoll^® has been used in the density gradient centrifugation isolation of intact chloroplasts from green alga, decidual stromal cells and distinct populations of rooster sperm.

Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used in cell separation and organelle isolation.

Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used to establish non-toxic low viscosity, low osmolarity density gradients that can be used to isolate cells, organelles and viruses. It has been used to separate inner and outer membrane vesicles from E. coli. Percoll is isosmotic throughout the gradient, non-toxic, and is easily removed from the purified materials.

Percoll is used in plant research for protoplast and organelle (chloroplast, mitochondria) separation.

Legal Information

Percoll is a registered trademark of Cytiva

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves