LY0100

Lysozyme Activity Kit

sufficient for 100 tests

• Synonym(s): Lysozyme assay kit
• NACRES: NA.26
• UNSPSC Code: 12352202

Properties

• usage: sufficient for 100 tests
• Quality Level: 200
• storage temp.: 2-8°C

Description

General description

The Lysozyme Detection Kit provides ready-to-use reagents for detecting the presence of lysozyme activity. This simple assay to detect lysozyme activity uses Micrococcus lysodeikticus cells as the substrate. Lysozyme activity results in the lysis of the Micrococcus lysodeikticus cells. During incubation of the lysozyme sample and substrate, the reaction is followed by monitoring the decrease in absorbance at 450 nm.

Application

Lysozyme Activity Kit has been used to measure the catalytic activity of native lysozyme, lysozyme purified from chicken egg white and lysozyme from pilocarpine-stimulated saliva.

Biochem/physiol Actions

Lysozyme hydrolytically cleaves the bonds between N-acetyl-D-glucosamine and N-acetylmuramic acid residues (GlcNAc β1-4Mur) in mucopolysaccharides and the glycan skeletons of the corresponding peptidoglycans (the latter are glycosaminoglycans linked to or crosslinked by peptide chains). Lysozyme effectively destroys the cell walls of bacteria.

Preparation Note

The Reaction Buffer is stable for at least 2 years at 2–8 °C.
The Lysozyme (Catalog Number L6876) may be stored long term at –20 °C and remains active for 4 years.
The Micrococcus lysodeikticus cells (Catalog Number M3770) should be stored long term at –20 °C.

Kit Components Also Available Separately

• L6876

  Lysozyme from chicken egg white, powder or granules, ≥39,000 units/mg protein

  SDS
• Lysozyme Reaction Buffer

  SDS
• M3770

  Micrococcus lysodeikticus ATCC No. 4698, suitable for substrate for the assay of lysozyme, lyophilized cells

  SDS

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable