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L5037

Sigma-Aldrich

Leptin from rat

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

Synonym(s):

OB

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About This Item

MDL number:
UNSPSC Code:
51111800
NACRES:
NA.32

recombinant

expressed in E. coli

Quality Level

sterility

non-sterile

Assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

≤0.8 ng/mL ED50

mol wt

16 kDa

impurities

≤1.0 EU/μg endotoxin

UniProt accession no.

shipped in

ambient

storage temp.

−20°C

Gene Information

rat ... Lep(25608)

Application

Leptin from rat has been used:
  • to check the effect of intranasal nerve growth factor (NGF) on NGF activity in the spinal cord of Sprague-Dawley rats
  • for validation of labelling for leptin-bearing cells of Sprague-Dawley rats
  • to study the effect of leptin on STAT3 (signal transducer and activator of transcription 3) phosphorylation in neural population of Sprague-Dawley rats
  • to study leptin response towards chemoreflex in nucleus of the solitary tract
  • in rats, to check the effect of association between leptin and neuronal nitric oxide pathway on penicillin-induced epileptiform activity

Biochem/physiol Actions

In rodents with uncontrolled diabetes mellitus, leptin is crucial for normalizing hyperglycemia. In the digestive tract, it also participates in the absorption of dietary molecules via controlling transporters activity.
Leptin is a hormone produced primarily in adipocytes, although leptin mRNA has also been identified in placenta and fetal tissues, gastric tissue and liver. Its primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight. Leptin increases the production of nitric oxide in endothelial cells and stimulates angiogenesis in vitro and in vivo.
Human and mouse leptin share ~84% sequence identity.

Physical form

Lyophilized from a 0.2 μm filtered solution with 4.5 mM NaHCO3.

Analysis Note

The biological activity is measured by its ability to induce proliferation of leptin-dependent human OB-R transfected murine BAF3 cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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John Ciriello et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 303(7), R727-R736 (2012-08-24)
Circulating levels of leptin are elevated in individuals suffering from chronic intermittent hypoxia (CIH). Systemic and central administration of leptin elicits increases in sympathetic nervous activity (SNA), arterial pressure (AP), and heart rate (HR), and it attenuates the baroreceptor reflex
Gregory J Morton et al.
The Journal of clinical investigation, 125(12), 4587-4591 (2015-11-04)
Leptin administration restores euglycemia in rodents with severe insulin-deficient diabetes, and recent studies to explain this phenomenon have focused on the ability of leptin to normalize excessive hypothalamic-pituitary-adrenal (HPA) axis activity. Here, we employed a streptozotocin-induced rat model (STZ-DM) of
P M Smith et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 296(3), R512-R520 (2008-11-21)
Adipose tissue plays a critical role in energy homeostasis, secreting adipokines that control feeding, thermogenesis, and neuroendocrine function. Leptin is the prototypic adipokine that acts centrally to signal long-term energy balance. While hypothalamic and brain stem nuclei are well-established sites
James W Maniscalco et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 306(8), R576-R585 (2014-02-14)
Leptin released peripherally acts within the central nervous system (CNS) to modulate numerous physiological and behavioral functions. Histochemical identification of leptin-responsive CNS cells can reveal the specific cellular phenotypes and neural circuits through which leptin signaling modulates these functions. Leptin
Luigi Aloe et al.
Neural regeneration research, 9(10), 1025-1030 (2014-09-11)
The purpose of this work was to investigate whether, by intranasal administration, the nerve growth factor bypasses the blood-brain barrier and turns over the spinal cord neurons and if such therapeutic approach could be of value in the treatment of

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