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Key Documents

HPA010871

Sigma-Aldrich

Anti-MUC3A antibody produced in rabbit

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Anti-Fragment, Anti-Intestinal mucin-3B, Anti-MUC-3B, Anti-Mucin-3B

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About This Item

UNSPSC Code:
12352203
Human Protein Atlas Number:
NACRES:
NA.43

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

product line

Prestige Antibodies® Powered by Atlas Antibodies

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

immunohistochemistry: 1:50- 1:200

immunogen sequence

PCPGTITITIVPASPTDPCVEMDPSTEATSPPTTPLTVFPFTTEMVTCPTSISIQTTLTTYMDTSSMMPESESSISPNASSSTGTGTVPTNTVFTSTRLPTSETWLSNSSVIPLPLPGVSTIPLTMKPSSS

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MUC3A(4584)

General description

MUC3A (mucin 3A) belongs to the family of epithelial mucins, which are both secreted as well as cell surface glycoproteins. This family is expressed by epithelial tissues, and MUC3A is predominantly expressed in the small intestine. This gene maps to human chromosome 7q22. MUC3A contains an extracellular region with two cysteine-rich EGF (epidermal growth factor)-like domains, intervened by an N-glycosylation-rich domain, along with a putative coiled-coil domain. This is succeeded by a transmembrane domain, and a cytoplasmic region consisting of 75 amino acids. MUC3A has an alternatively spliced isoform which contains the first EGF- like domain, but lacks the second EGF-like domain and the transmembrane region.

Immunogen

Mucin-3B recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information.

Biochem/physiol Actions

Mucin family of proteins is involved in epithelial cell protection, regulation of adhesion and cell signaling. MUC3A (mucin 3A) confers protection to intestinal epithelial cells against the external environment. Variations in this gene determine the susceptibility of an individual to ulcerative colitis and Crohn′s disease (CD). This protein is expressed in synovial lining cells, macrophages, and fibroblasts, and it is overexpressed in synovial tissue. Therefore, it might play a role in the pathogenesis of arthritis. Hypomethylation of the 5′-flanking region of MUC3A gene determines the expression of this gene in various cancers such as breast, pancreas, colon and lung cancer cell lines. Therefore, methylation studies of this gene might help in predicting cancer risk as well prognosis of cancer patients. This gene is also up-regulated in renal clear cell carcinoma, and is involved in tumorigenesis of renal cancer.

Features and Benefits

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST70010

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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S J Williams et al.
Biochemical and biophysical research communications, 261(1), 83-89 (1999-07-16)
Epithelial mucins are a family of secreted and cell surface glycoproteins expressed by epithelial tissues and implicated in epithelial cell protection, adhesion modulation and signaling. The gene encoding human MUC3 (hMUC3), localised to chromosome 7q22, is most highly expressed in
Michael V Volin et al.
Arthritis and rheumatism, 58(1), 46-52 (2008-01-01)
Rheumatoid arthritis (RA) is a chronic inflammatory disease that is characterized by hypertrophy of the synovial tissue, leukocyte infiltration, angiogenesis, and ultimately joint destruction. Mucins (MUCs) are a family of heavily glycosylated proteins that protect epithelial membranes and are used
Xavier Leroy et al.
Urology, 62(4), 771-775 (2003-10-11)
To investigate, by real-time quantitative reverse transcriptase-polymerase chain reaction, the expression of MUC3 and vascular endothelial growth factor (VEGF) to correlate them with histologic parameters and with prognosis. Human mucins are large O-glycoproteins expressed in epithelial tissues. Deregulation of mucin
K Kyo et al.
Journal of human genetics, 46(1), 5-20 (2001-04-06)
Ulcerative colitis (UC) and Crohn's disease (CD), the major forms of inflammatory bowel diseases (IBDs). are multifactorial disorders of unknown etiology. We reported a possible association of rare variable number of tandem repeat (VNTR) alleles of the "MUC3" gene with
Sho Kitamoto et al.
Biochemical and biophysical research communications, 397(2), 333-339 (2010-06-01)
MUC3A is a membrane-bound glycoprotein that is aberrantly expressed in carcinomas and is a risk factor for a poor prognosis. However, the exact mechanism of MUC3A expression has yet to be clarified. Here, we provide the first evidence that MUC3A

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