D3196

Dipeptidyl Peptidase VIII human

• NACRES: NA.54
• UNSPSC Code: 12352204
• Specific activity: ≥20 units/μg protein
• Recombinant: expressed in Sf9 cells
• Concentration: ≥0.08 mg/mL

Properties

• Quality Segment: 200
• recombinant: expressed in Sf9 cells
• form: solution
• specific activity: ≥20 units/μg protein
• mol wt: 100 kDa
• concentration: ≥0.08 mg/mL
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Full length sequence with an N-terminal GST tag

Application

Human dipeptidyl peptidase VIII has been used in a study to identify ERGIC-53 as an intracellular transport receptor of α1-antitrypsin. Human dipeptidyl peptidase VIII has also been used in a study to investigate the cargo selectivity of the ERGIC-53/MCFD2 transport receptor complex.

Biochem/physiol Actions

Dipeptidyl peptidase VIII (DPP8) is a 100 kDa monomeric protein expressed in the cytoplasm. It is a postproline dipeptidyl aminopeptidase that is homologous to DPPIV and fibroblast activation protein (FAP). It hydrolyzes Ala-Pro, Arg-Pro and Gly-Pro. DPP8 has a neutral pH optimum. It may be involved in T-cell activation and immune function as it is similar to DPPIV . It is upregulated during immune activation.

Physical form

Supplied as a solution in 40 mM Tris-HCL, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT and 20% glycerol.

Other Notes

One unit will hydrolyze 1.0 picomole of Ala-Pro-AMC per minute at pH 7.4 at 25 deg °C

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1