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Key Documents

DMS 454

95062832, human lung, Polygonal

Synonym(s):

DMS454 Cells

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About This Item

UNSPSC Code:
41106514

product name

DMS 454, 95062832

biological source

human lung

growth mode

Adherent

karyotype

Not specified

morphology

Polygonal

products

Calcitonin

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human Caucasian lung, small cell carcinoma

Cell Line Description

The small cell carcinoma DMS 454 was isolated in 1981 from lung tissue of a male patient at time of autopsy. The cells contain dense-core vesicles and secrete calcitonin.

Application

Human lung tumour model, hormone secretion

Culture Medium

Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (heat-inactivated).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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