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RAW 264 Cell Line from mouse

85062803, mouse blood, Macrophage

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About This Item

UNSPSC Code:
41106514

product name

RAW 264 Cell Line from mouse, 85062803

biological source

mouse blood

description

Mouse leukemic monocyte-macrophage

growth mode

Semi-adherent

karyotype

Not specified

morphology

Macrophage

products

Lysozyme

receptors

Immunoglobulin and complement

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse leukaemic monocyte-macrophage

Cell Line Description

Established from an ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia virus (A-MuLV). Cells with pinocytose neutral red and phagocytose zymosan. Cells capable of antibody-dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS (0.5ng/ml).

Application

RAW 264 Cell Line from mouse has been used:
  • to study the anti-inflammatory effect of galectin-3 on RAW 264 macrophages which are stimulated with lipopolysaccharide
  • to study the effect of titanium particles on progranulin (PGRN) expression in RAW 264 cells
  • to study the effects of a nutritional supplement on interferon-γ (IFN-γ)-induced expression of macrophage chemoattractant protein-1 (MCP-1) and intercellular adhesion molecule-1 (ICAM-1)

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2; 5% CO2; 37°C. Remove the cells mechanically. Cells are semi-adherent, i.e. some cells grows in suspension, some loosely attach to the surface and others flattened out and attach to the flask. Cells should not be allowed to overgrow and become confluent as this can lead to loss of the flattened adherent cell characteristic.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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