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C9409

Creatinine Deiminase microbial

lyophilized powder, ≥25 units/mg protein

Synonym(s):

Creatinine Deaminase

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About This Item

CAS Number:
37289-15-9
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-449-1
MDL number:
MFCD00130878
EC Number:
3.5.4.21 (BRENDA, IUBMB)
Specific activity:
≥25 units/mg protein
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form

lyophilized powder

Quality Segment

200

specific activity

≥25 units/mg protein

mol wt

~260 kDa

composition

Protein, ≥15% biuret

storage temp.

−20°C

General description

Isoelectric point : 4.4
Michaelis constant : 3.5 x 10‾3M (Creatinine)
Structure : 6 subunits per mol of enzyme
Inhibitors : Ag+,Hg++, o-phenanthroline,monoiodoacetate
Optimum pH : 8.5 – 9.5
Optimum temperature : 65 – 75°C
pH Stability : pH 7.0 – 11.0 (30°C, 20hr)
Thermal stability : Below 65°C (pH 7.5, 1hr)

Application

Creatinine Deiminase microbial has been used:
  • to immobilize aminosilylated glass beads based biosensor for ammonia/ammonium and creatinine detection in urine
  • in creating creatinine-sensing membrane for biophysical studies
  • to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples

Creatinine deiminase has been used in a study to assess the application of a creatinine-sensitive biosensor for hemodialysis control. Creatinine deiminase has also been used in a study to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples.

Biochem/physiol Actions

Creatinine deiminase catalyzes the hydrolysis of creatinine to methylhydantoine and ammonia.

Physical form

Lyophilized powder containing mannitol as stabilizer

Other Notes

One unit will hydrolyze 1.0 μmole of creatinine to N-methylhydantoin and NH3 per min at pH 7.5 at 37 °C in a coupled system with L-glutamic dehydrogenase.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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