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Key Documents

B3640

Sigma-Aldrich

Anti-Biotin antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym(s):

Anti-Biotin, Anti Biotin Antibody, Anti Biotin Antibody - Anti-Biotin antibody produced in goat, Anti-Biotin Antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

technique(s)

immunoelectrophoresis: suitable

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

Biotin is considered as an essential nutrient, though it is know to be secreted by some of the intestinal bacteria. It is found in liver and brain tissues in minute quantities.
Biotin:avidin second antibody signal systems involve the conjugation of biotin to a secondary antibody. Once the secondary antibody binds to a target primary antibody in an immunochemical assay, the signal is developed by allowing avidin which has been conjugated to an enzyme or signal molecule to bind to the antibody associated biotin molecule.
Specificity and reactivity are determined by Ouchterlony Double Diffusion and immunoelectrophoresis versus the biotin-carrier protein complex. No reactivity is observed versus the carrier protein.

Immunogen

Biotin-KLH conjugate

Application

Anti-Biotin antibody produced in goat has been used in multi-well plate assay, to identify desthiobiotinylating (analog of biotin) proteins. It has also been used in Colourimetric enzyme immunoassay.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

Biochem/physiol Actions

Biotin serves as a cofactor for carboxylases, necessary for oxidative metabolism. Biotin absorption in small intestine is reduced on consumption of avidin rich food such as egg white. Thus, greatly decreasing its bioavailability, leading to egg white injury. Reduced levels of biotin is normally observed during pregnancy. Biotin deficiency might be congenital, with defective proteins involved in biotin homeostasis.

Physical form

Lyophilized from 0.01 M sodium phosphate, 15 mM sodium chloride, pH 7.2

Reconstitution

Reconstitute with 0.135 M sodium chloride.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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