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Merck

A5757

Sigma-Aldrich

Antifoam B Emulsion

aqueous-silicone emulsion

Synonym(s):

Antifoaming agent B

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About This Item

UNSPSC Code:
12161900
NACRES:
NA.25

Pricing and availability is not currently available.

Quality Level

form

emulsion

contains

emulsifier (different from those present in Antifoam A emulsion)

technique(s)

cell culture | hybridoma: suitable
microbiological culture: suitable

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This Item
HPA038642HPA066105HPA048581
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

product line

Prestige Antibodies® Powered by Atlas Antibodies

product line

Prestige Antibodies® Powered by Atlas Antibodies

product line

Prestige Antibodies® Powered by Atlas Antibodies

product line

Prestige Antibodies® Powered by Atlas Antibodies

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

technique(s)

immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:20-1:50

technique(s)

immunohistochemistry: 1:500- 1:1000

technique(s)

immunofluorescence: 0.25-2 μg/mL

technique(s)

immunohistochemistry: 1:50- 1:200

General description

Antifoam eliminates excessive foaming formed during impingement, due to proteins and/or carbohydrates in the collection medium. Antifoam B emulsion is a silicone based antifoam.[1] Antifoam B emulsion does not affect the growth of bacteria.[2] Antifoam B emulsion functions as an antifoaming agent in shake flask culture of bacteria.[3]

Application

Antifoam B emulsion has been used:
  • to enhance collection efficiency in collection media[1]
  • as a component in stress granule lysis buffer to reduce foaming[4], in host cell lysis buffer to reduce foaming caused by detergents[2]
  • to improve solubility of antibiotics[5]
  • as a foaming controls in bacterial growth media[3]

Other Notes

A 10% aqueous emulsion of polydimethylsiloxane

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

>214.0 °F - closed cup

Flash Point(C)

> 101.1 °C - closed cup


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