Preparing a fresh solution and replenishing the medium frequently is advisable. If frequent feeding is not feasible, switching to a stable analog, e.g., Ascorbic Acid 2-Phosphate (A8960), is highly recommended.
A4403
L-Ascorbic acid
powder, suitable for cell culture, γ-irradiated
Synonym(s):
L-Threoascorbic acid, Antiscorbutic factor, Vitamin C
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About This Item
Linear Formula:
C6H8O6
CAS Number:
Molecular Weight:
176.12
UNSPSC Code:
12352207
NACRES:
NA.75
PubChem Substance ID:
EC Number:
200-066-2
Beilstein/REAXYS Number:
84272
MDL number:
Form:
powder
Pricing and availability is not currently available.
biological source
synthetic (organic)
Quality Segment
sterility
γ-irradiated
form
powder
mol wt
Mw 176.12 g/mol
technique(s)
cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable
color
white to off-white
pH
1.0-2.5 (25 °C, 176 g/L in water)
mp
190-194 °C (dec.)
solubility
H2O: 10 mg/mL
storage temp.
room temp
SMILES string
OC([C@]([C@@H](O)CO)([H])O1)=C(O)C1=O
InChI
1S/C6H8O6/c7-1-2(8)5-3(9)4(10)6(11)12-5/h2,5,7-10H,1H2/t2-,5+/m0/s1
InChI key
CIWBSHSKHKDKBQ-JLAZNSOCSA-N
Gene Information
human ... SLC23A2(9962)
General description
Listen to an Audio Overview
L-ascorbic acid is an unsaturated γ-lactone ring containing an enediol configuration (–COH=COH) attached with a carbonyl group.
L-ascorbic acid is an unsaturated γ-lactone ring containing an enediol configuration (–COH=COH) attached with a carbonyl group.
Application
For use in cell culture applications as an antioxidant and to regenerate tocopherol.
L-ascorbic acid has been used:
L-ascorbic acid has been used:
Biochem/physiol Actions
L-ascorbic acid mainly exhibits antioxidant properties. It protects plants from oxidative stress and mammals from diseases associated with oxidative stress. L-ascorbic acid mainly protects from hydroxyl radicals, superoxide and singlet oxygen. In addition, it also reduces the membrane-linked antioxidant α-tocopherol (oxidised form). L-Ascorbic acid enhances endothelium-dependent vasodilation in various disorders, including diabetes, coronary artery disease, hypertension and chronic heart failure.
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This Item | |||
|---|---|---|---|
| description powder, suitable for cell culture, γ-irradiated | description reagent grade, crystalline | description analytical standard | description FCC, FG |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 300 |
| form powder | form crystalline | form solid | form powder or crystals, crystalline |
| pH 1.0-2.5 (25 °C, 176 g/L in water) | pH 1.0-2.5 (25 °C, 176 g/L in water) | pH 1.0-2.5 (25 °C, 176 g/L in water) | pH 1.0-2.5 (25 °C, 176 g/L in water) |
| storage temp. room temp | storage temp. room temp | storage temp. 2-8°C | storage temp. - |
| solubility H2O: 10 mg/mL | solubility water: soluble 50 mg/mL | solubility water: soluble 176 g/L at 20 °C | solubility water: soluble 176 g/L at 20 °C |
| mp 190-194 °C (dec.) | mp 190-194 °C (dec.) | mp 190-194 °C (dec.) | mp 190-194 °C (dec.) |
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Storage Class
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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How can the stability of L-ascorbic acid be maintained and its oxidation prevented in cell culture, given that the cells are consistently exposed to air and oxygen?. What methods can be used to prevent it in cell culture under oxygen-rich conditions?
1 answer-
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Do you happen to have any information on how long L-ascorbic acid remains stable at 37°C? Do you have any idea after how much time the full activity of this compound is lost?
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While stability studies at 37°C have not been conducted, this product ships at ambient temperature and is fairly robust in the powder form. Solutions tend to degrade and lose activity fairly rapidly in the presence of oxygen and at alkaline pH. Additional information is available in the product datasheet available for L-Ascorbic Acid, product A7506:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/221/043/a7506pis.pdfHelpful?
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What is the right storage condition for the powder upon its arrival?
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This product may be stored at room temperature in powder form.
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What is the recommended storage method for stock solutions of item A4403, L-Ascorbic acid?
1 answer-
Stock solutions of item A4403, L-Ascorbic acid, can be stored at -20°C for long-term (months to years) stability.
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In what solvents is A4403 soluble?
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The product A4403 is soluble in water at a concentration of 10 mg/ml, resulting in a clear solution.
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