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1,3,5-Triphenyltetrazolium formazan

Name: 1,3,5-Triphenyltetrazolium formazan
Brand: SIGMA

≥90% (UV)

Synonym(s):

TTC formazan

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About This Item

Empirical Formula (Hill Notation):

C₁₉H₁₆N₄

CAS Number:

531-52-2

Molecular Weight:

300.36

UNSPSC Code:

12352204

NACRES:

NA.83

PubChem Substance ID:

57653521

EC Number:

208-509-1

Beilstein/REAXYS Number:

753057

MDL number:

MFCD00020647

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Properties

Quality Segment

100

assay

≥90% (UV)

form

powder

solubility

dioxane: 50 mg/mL, clear

SMILES string

N(\N=C(/N=N/c1ccccc1)c2ccccc2)c3ccccc3

InChI

1S/C19H16N4/c1-4-10-16(11-5-1)19(22-20-17-12-6-2-7-13-17)23-21-18-14-8-3-9-15-18/h1-15,20H/b22-19-,23-21+

InChI key

BEIHVSJTPTXQGB-YMGPPEANSA-N

Description

General description

1,3,5-Triphenyltetrazolium formazan is an insoluble compound produced upon the reduction of 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) salt, which is an indicator of cell redox potential. This reaction is irreversible. TTC formazan is brightly colored and can be quantified using a spectrophotometer.

Application

1,3,5-Triphenyltetrazolium formazan has been used:

for the quantification of 2,3,5-triphenyltetrazolium chloride (TTC) reduction activity in bacterial cells
as a standard to study the optical density of formazan produced by TTC-treated milled seeds
to stain heart tissue for infarct size assessment or undergo perfusion fixation to allow morphometric studies in addition to histology and immunohistochemistry

Other Notes

Review: Tetrazolium salts and formazans

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This Item	M2003	88417	T8877
Sigma-Aldrich 93145 1,3,5-Triphenyltetrazolium formazan	Sigma-Aldrich M2003 MTT Formazan	Sigma-Aldrich 88417 MTT Formazan	Sigma-Aldrich T8877 2,3,5-Triphenyltetrazolium chloride
description ≥90% (UV)	description powder	description MTT reduction end product	description ≥98.0% (HPLC)
assay ≥90% (UV)	assay ≥97% (TLC)	assay -	assay ≥98.0% (HPLC)
solubility dioxane: 50 mg/mL, clear	solubility acetone: 1 mg/mL	solubility chloroform: 10 mg/mL, clear, dark violet	solubility H₂O: 50 mg/mL
Quality Level 100	Quality Level 200	Quality Level 100	Quality Level 200
form powder	form powder	form powder	form powder
TTC formazan 1,3,5-Triphenyltetrazolium formazan	-	-	-