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P8074

Sigma-Aldrich

TWEEN® 80

BioXtra, viscous liquid

Synonym(s):

TWEEN® 80, POE (20) sorbitan monooleate, Polyethylene glycol sorbitan monooleate, Polyoxyethylenesorbitan monooleate, Polysorbate 80

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About This Item

CAS Number:
9005-65-6
MDL number:
MFCD00082107
UNSPSC Code:
12161900
NACRES:
NA.21
Pricing and availability is not currently available.

description

non-ionic

Quality Level

200

product line

BioXtra

form

viscous liquid

mol wt

micellar avg mol wt 79,000
average mol wt 1310

composition

Oleic acid, ~70% (balance primarily linoleic, palmitic, and stearic acids)

aggregation number

60

technique(s)

DNA extraction: suitable
RNA extraction: suitable
cell culture | mammalian: suitable
protein purification: suitable

impurities

≤0.025% Phosphorus (P)

ign. residue

<0.1%

CMC

0.012 mM (20-25°C)

transition temp

cloud point 65 °C

density

1.06 g/mL at 25 °C

anion traces

chloride (Cl-): <0.05%
sulfate (SO42-): <0.05%

cation traces

Al: <0.0005%
Ca: <0.001%
Cu: <0.0005%
Fe: <0.0005%
K: <0.005%
Mg: <0.0005%
NH4+: <0.05%
Na: <0.05%
Pb: <0.001%
Zn: <0.0005%

HLB

15

SMILES string

CCCCCCCC/C=C/CCCCCCCC(=O)OCCOCC(C1C(CC(O1)OCCO)OCCO)OCCO

InChI

1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+

InChI key

RGPBUVUVZKQNHD-MDZDMXLPSA-N

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General description

["TWEEN"] 80 is a polyethylene sorbitol ester, broadly used in biochemical applications like solubilizing proteins, isolating nuclei from cells in culture, and growing tubercle bacilli. It is also useful in emulsifying and dispersing substances in medicinal and food products. TWEEN 80 acts as an active drying agent in ice cream.

Application

["TWEEN"] 80 has been used:
  • to dissolve rapamycin (RAPA) for immunosuppressive treatment to perform in vivo studies in mice
  • to dissolve remodelin and remodelin Fluor to assess remodelin toxicity in vivo and small molecule dosing
  • to prepare assay medium for the microbiological assay for total folate

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

>235.4 °F - closed cup

Flash Point(C)

> 113 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL8873
BCCJ7552
BCCH2519
BCCF5441
BCCD6595

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Brian Hassett et al.
mAbs, 10(1), 166-176 (2017-10-12)
Fusion protein and monoclonal antibody-based tumor necrosis factor (TNF) inhibitors represent established treatment options for a range of inflammatory diseases. Regulatory authorities have outlined the structural characterization and clinical assessments necessary to establish biosimilarity of a new biotherapeutic product with
Geoffrey D Keeler et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 26(1), 173-183 (2017-09-26)
The devastating neurodegenerative disease multiple sclerosis (MS) could substantially benefit from an adeno-associated virus (AAV) immunotherapy designed to restore a robust and durable antigen-specific tolerance. However, developing a sufficiently potent and lasting immune-regulatory therapy that can intervene in ongoing disease
Joel Selkrig et al.
Scientific reports, 8(1), 10646-10646 (2018-07-15)
In animals, commensal microbes modulate various physiological functions, including behavior. While microbiota exposure is required for normal behavior in mammals, it is not known how widely this dependency is present in other animal species. We proposed the hypothesis that the
Sen Zhu et al.
Oncogene, 39(1), 17-29 (2019-08-30)
B lymphoma Mo-MLV insertion region 1 (BMI1) has been reported to be an oncoprotein. BMI1 represses tumor suppressors to promote cell proliferation, epithelial-mesenchymal transition (EMT), and cancer progression. Although it is known that the expression of BMI1 is increased in
Praneeth Reddy Devulapally et al.
Genome medicine, 10(1), 34-34 (2018-04-29)
Existing methods for paired antibody heavy- and light-chain repertoire sequencing rely on specialized equipment and are limited by their commercial availability and high costs. Here, we report a novel simple and cost-effective emulsion-based single-cell paired antibody repertoire sequencing method that
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Articles

Workflow for the Analysis of Polysorbate 80 in Erbitux® Formulation

A complete reversed phase HPLC-ELSD workflow, including sample preparation for the analysis of Polysorbate 80 in a monoclonal antibody formulation.

Questions

1–3 of 3 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. What’s the concentration of P8074-100ml Tween 80? Thank you.

    1 answer

    1. This Tween 80 product is the pure form, it has not been diluted in water or other aqueous buffers. The composition of this surfactant is available in the PROPERTIES section of the product detail page and is approximately 70 % oleic acid with the balance being primarily linoleic, palmitic, and stearic acids.

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