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MAB1522

Sigma-Aldrich

Anti-Actin Antibody, smooth muscle γ & α actin, clone CGA7

ascites fluid, clone CGA7, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

clone

CGA7, monoclonal

species reactivity

human, rat, chicken, rabbit, monkey

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG2a

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Specificity

Antibody recognizes actin isotypes alpha and gamma of smooth muscle cells, but not skeletal or cardiac muscle cells. Reactive with myoepithelium, but nonreactive with other mesenchymal and all epithelial cells. The antibody labels eiomyosarcomas and leiomyomas but does not react with other neoplasms such as carcinomas or lymphoma.

Immunogen

Chicken gizzard actin
Epitope: smooth muscle gamma & alpha actin

Application

Detect Actin using this Anti-Actin Antibody, smooth muscle γ & α actin, clone CGA7 validated for use in WB, IH, IH(P).
Western Blot: 1:1000 (approximately 43 kDa)

Immunohistochemistry (paraffin, formalin, deparaffinized methacarn-fixed tissue: 1:10,000 or alcohol fixation)

Optimal dilutions must be determined by the end user.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Clinical cancer research : an official journal of the American Association for Cancer Research, 13(1), 161-169 (2007-01-04)
Intratumoral microvascular density (MVD) has been controversial as an indicator of prognosis in clear cell renal cell carcinoma (CCRCC). Classification of the intratumoral blood vessels based on differential expressions of blood vessel markers has not been correlated with patient prognosis
Cultured circular smooth muscle from the rabbit colon.
Kao, H W, et al.
In Vitro Cellular & Developmental Biology, 24, 787-794 (1988)
Functional expression of the GABAB receptor in human airway smooth muscle.
Osawa, Y; Xu, D; Sternberg, D; Sonett, JR; D'Armiento, J; Panettieri, RA; Emala, CW
American Journal of Physiology. Lung Cellular and Molecular Physiology null
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Receptor-operated Ca2+ entry mediated by TRPC3/TRPC6 proteins in rat prostate smooth muscle (PS1) cell line.
S Thebault, A Zholos, A Enfissi, C Slomianny, E Dewailly, M Roudbaraki, J Parys, Natalia Prevarskaya
Journal of Cellular Physiology null

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