1.50256
Purospher® STAR RP-18 endcapped (5 µm) HPLC Columns
L × I.D. 250 mm x 2 mm LiChroCART™ HPLC cartridge, manuCART™ holder required for use
Synonym(s):
Purospher® STAR RP-18 endcapped (5 µm) LiChroCART™ 250-2
About This Item
Recommended Products
product name
Purospher® STAR RP-18 endcapped (5 µm), L × I.D. 250 mm x 2 mm LiChroCART™ HPLC cartridge, manuCART™ holder required for use
material
stainless steel column
Quality Level
Agency
suitable for USP L1
product line
Purospher®
feature
endcapped
packaging
1 ea of
extent of labeling
17% Carbon loading
parameter
250 bar pressure
65 °C max. temp.
technique(s)
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
column L × I.D.
25 cm × 2 mm
surface area
330 m2/g
matrix
fully porous particle
matrix active group
C18 bonding phase
particle size
5 μm
pore size
120 Å pore size
operating pH range
1.5-10.5
separation technique
reversed phase
storage temp.
no temp limit
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General description
These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test.
Purospher® STAR RP-18 endcapped, 5 µm LiChroCART™ 250-2 is a cartridge column which requires a manuCART™ cartridge column holder part number 1.51486.0001. The manuCART™ can be used to hold one cartridge column with or without direct integration of a guard column.
LiChroCART™ cartridge columns mounted with a manuCART™ cartridge column holder are hand-tight only and have therefore a maximum pressure stability of 250 bar.
For higher pressure stability requirements Purospher® STAR RP-18 endcapped, 5 µm Hibar® RT 250-2 HPLC columns are available part number 1.50598.0001.
For use under UHPLC conditions (where a pressure stability up to 1000 bar is required) it is recommended to use a Purospher® STAR RP-18 endcapped, 3 µm Hibar® HR 250-2.1 UHPLC Column part number 1.50655.0001
The column dimension of 250 mm length x 2 mm I.D. is the optimal choice for HPLC separation of complex samples at lower flow rate, allowing significant solvent savings and improved sensitivity
Learn more about Purospher® STAR HPLC and UHPLC Columns
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide
Application
Features and Benefits
- Ultra-pure silica (99.999%) for excellent peak symmetry
- High separation efficiency
- Reproducible results from run-to-run and batch-to-batch
- Best all-round performance according to Tanaka
- Outstanding pH stability from pH 1.5 to 10.5
- No phase collapse when using highly aqueous mobile phases
- Good suitability for LC-MS applications
Analysis Note
Theoretical Plates (N/m) (hexylbenzene): ≥ 70000
Symmetry (hexylbenzene): 0.8 - 1.4
Capacity factor (hexylbenzene): ≥ 8.0
Legal Information
Storage Class Code
11 - Combustible Solids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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