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1.50251

Supelco

Purospher® STAR RP-18 endcapped (5 µm) HPLC Columns

L × I.D. 125 mm x 4 mm LiChroCART® HPLC cartridge, manuCART holder required for use

Synonym(s):

Purospher® STAR RP-18 endcapped (5 µm) LiChroCART® 125-4

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52

product name

Purospher® STAR RP-18 endcapped (5 µm), L × I.D. 125 mm x 4 mm LiChroCART® HPLC cartridge, manuCART holder required for use

material

stainless steel column

Quality Level

Agency

suitable for USP L1

product line

Purospher®

feature

endcapped

packaging

1 ea of

extent of labeling

17% Carbon loading

parameter

250 bar pressure
65 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

12.5 cm × 4 mm

surface area

330 m2/g

matrix

fully porous particle

matrix active group

C18 bonding phase

particle size

5 μm

pore size

120 Å pore size

operating pH range

1.5-10.5

separation technique

reversed phase

storage temp.

no temp limit

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General description

Purospher® STAR RP-18 endcapped, 5 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Purospher® STAR RP-18 endcapped, 5 µm LiChroCART® 125-4 is a cartridge column which requires a manuCART cartridge column holder part number 1.51486.0001. The manuCART can be used to hold one cartridge column with or without direct integration of a 4-4 mm guard column. The recommended guard column is Purospher® STAR RP-18 endcapped (5 µm) LiChroCART® 4-4 part number 1.50250.0001. LiChroCART® cartridge columns mounted with a manuCART cartridge column holder are hand-tight only and have therefore a maximum pressure stability of 250 bar. For higher pressure stability requirements Purospher® STAR RP-18 endcapped, 5 µm Hibar® RT 125-4 HPLC columns are available part number 1.50036.0001.The column dimension of 125 mm length x 4 mm I.D. is the optimal choice for HPLC separation of complex samples.Item 1.50251.1003 is a Validation kit containing 3 Purospher® STAR RP-18 endcapped, 5 µm LiChroCART® 125-4 cartridge columns from 3 different base material lots.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.

Features and Benefits

  • Ultra-pure silica (99.999%) for excellent peak symmetry
  • High separation efficiency
  • Reproducible results from run-to-run and batch-to-batch
  • Best all-round performance according to Tanaka
  • Outstanding pH stability from pH 1.5 to 10.5
  • No phase collapse when using highly aqueous mobile phases
  • Good suitability for LC-MS applications

Analysis Note

Pressure: ≤ 65 bar
Theoretical Plates (N/m) (Toluene): ≥ 80000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): ≥ 1.5

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany
MANUCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Discover the Tanaka Test, a key protocol for evaluating the performance and selectivity of stationary phases in Reversed Phase HPLC separations.

Discover the Tanaka Test, a key protocol for evaluating the performance and selectivity of stationary phases in Reversed Phase HPLC separations.

Discover the Tanaka Test, a key protocol for evaluating the performance and selectivity of stationary phases in Reversed Phase HPLC separations.

Discover the Tanaka Test, a key protocol for evaluating the performance and selectivity of stationary phases in Reversed Phase HPLC separations.

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