Solutions can be sterile filtered using 0.2 μm filters (low protein binding). Do not autoclave solutions.
T1895
Thymidine
powder, BioReagent, suitable for cell culture
Synonym(s):
1-(2-Deoxy-β-D-ribofuranosyl)-5-methyluracil, 1-(2-Deoxy-β-D-ribofuranosyl)thymine, 2′-Deoxythymidine, Thymine deoxyriboside, dT
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About This Item
Empirical Formula (Hill Notation):
C10H14N2O5
CAS Number:
Molecular Weight:
242.23
UNSPSC Code:
12352207
NACRES:
NA.75
PubChem Substance ID:
EC Number:
200-070-4
Beilstein/REAXYS Number:
89285
MDL number:
Assay:
≥99%
Biological source:
synthetic (organic)
Form:
powder
Solubility:
water: 50 mg/mL, clear, colorless
Pricing and availability is not currently available.
biological source
synthetic (organic)
Quality Segment
product line
BioReagent
assay
≥99%
form
powder
technique(s)
cell culture | mammalian: suitable
mp
186-188 °C (lit.)
solubility
water: 50 mg/mL, clear, colorless
SMILES string
CC1=CN([C@H]2C[C@H](O)[C@@H](CO)O2)C(=O)NC1=O
InChI
1S/C10H14N2O5/c1-5-3-12(10(16)11-9(5)15)8-2-6(14)7(4-13)17-8/h3,6-8,13-14H,2,4H2,1H3,(H,11,15,16)/t6-,7+,8+/m0/s1
InChI key
IQFYYKKMVGJFEH-XLPZGREQSA-N
Gene Information
human ... DTYMK(1841)
General description
Thymidine is also referred to as pyrimidine deoxynucleoside. Deoxythymidine is a nucleoside present in DNA. In a DNA double stranded structure, thymidine pairs with deoxyadeninosine.
Application
Thymidine can be used in HT and HAT cocktails for hybridoma fusion, selection and cloning. It has been used:
- as a nucleotide source in minimal essential medium to evaluate the outgrowth of the iSCNT-derived blastocysts
- as a supplement in Dulbecco′s Modified Eagle Medium (DMEM) selection medium used for the production of anti-neurogranin (NRGN) antibodies
- to prepare THMG (thymidine, hypoxanthine, glycine, methotrexate) and THG stock solution for cleansing mouse lymphoma cells
Biochem/physiol Actions
Thymidine is useful in cell synchronization during S-phase. In the salvage pathway of pyrimidines, pyrimidine phosphorylase reversibly converts thymine to thymidine.
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This Item | |||
|---|---|---|---|
| biological source synthetic (organic) | biological source synthetic (organic) | biological source - | biological source synthetic |
| Quality Level 300 | Quality Level 300 | Quality Level 100 | Quality Level 200 |
| form powder | form powder | form solid | form powder |
| assay ≥99% | assay ≥99% | assay ≥98% (HPLC) | assay ≥99.0% (HPLC) |
| solubility water: 50 mg/mL, clear, colorless | solubility water: 50 mg/mL, clear, colorless | solubility water: 10 mg/mL | solubility hot water: 1 g/10 mL |
| mp 186-188 °C (lit.) | mp 186-188 °C (lit.) | mp - | mp 185-190 °C, 186-188 °C (lit.) |
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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How can I sterilize a solution of Product T1895, Thymidine?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How can I solubilize Product T1895, Thymidine?
1 answer-
This product is soluble in water (50 mg/mL), yielding a clear, colorless solution with heating. For aqueous concentrations higher than 50 mg/mL, solubility can be enhanced with the addition of a small amount of NaOH solution. Add the base drop wise with slow stirring. Thymidine is also soluble in methanol, hot alcohol, hot acetone, hot ethyl acetate, pyridine, glacial acetic acid, and is sparingly soluble in hot chloroform.
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How long can I store media supplemented with Product T1895, Thymidine?
1 answer-
Thymidine is a component of Nutrient Mixture F-12 at 0.73mg/L. This media has an expiration date of 18 months from date of formulation. You will need to determine if this is appropriate for your application.
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What concentration of Product T1895, Thymidine, is used for testing this product for cell culture applications?
1 answer-
The tested working concentration for cell culture for Thymidine is 8 mg/L.
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