T0910

Thioredoxin from Escherichia coli

recombinant, expressed in E. coli, essentially salt-free, lyophilized powder, ≥3 units/mg protein

• CAS Number: 52500-60-4
• UNSPSC Code: 12352200
• NACRES: NA.32
• MDL number: MFCD00132423
• Form: essentially salt-free, lyophilized powder
• Assay: ≥90% (SDS-PAGE)
• Biological source: Escherichia coli
• Recombinant: expressed in E. coli
• Mol wt: 11.7 kDa

Properties

• biological source: Escherichia coli
• Quality Segment: 200
• recombinant: expressed in E. coli
• assay: ≥90% (SDS-PAGE)
• form: essentially salt-free, lyophilized powder
• specific activity: ≥3 units/mg protein
• mol wt: 11.7 kDa
• technique(s): activity assay: suitable
• solubility: water: 1.90-2.10 mg/mL, clear, colorless
• Protein ID accession no.: M26133
• UniProt accession no.: P0AA25
• application(s): cell analysis
• storage temp.: −20°C
• Gene Information: Escherichia coli K12 ... trxA(948289)

Description

General description

Research area: Cell Signaling

Thioredoxin from Escherichia coli is a monomeric, globular protein. It has a central β-sheet surrounded by α-helices.

Application

Thioredoxin from Escherichia coli has been used in thioredoxin reductase assay, peroxidase-coupled thioredoxin system assay and peroxiredoxin assay.[1][2] It has also been used to study its effects on photoreceptor death in a mouse model of retinitis pigmentosa (RP) and its potential to modify retinal oxidative stress, glial changes, and vascular changes during the progression of the disease.

Biochem/physiol Actions

Mediates the reduction of disulfide bonds in proteins.

Thioredoxin from Escherichia coli catalyzes REDOX reactions and has a role in various biological processes. It modulates the structure and activity of many proteins. The enzyme thioredoxin reductase catalyzes the reduction of thioredoxin. Thioredoxin also has a role in oxidative stress response.Thioredoxin, known as both adult T cell leukemia-derived factor (ADF) and a cellular redox enzyme, functions as a cytokine or chemokine, facilitating cell-to-cell communication. Additionally, it plays a role in regulating cell growth and apoptosis.

Other Notes

GenBank^® Accession Number M26133.

One unit will cause a ΔA₆₅₀ of 1.0 in 1 min at 25 °C in the insulin reduction assay.

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)