SML1177

ML323

≥98% (HPLC)

• Synonym(s): N-(4-(1H-1,2,3-Triazol-1-yl)benzyl)-2-(2-isopropylphenyl)-5-methylpyrimidin-4-amine
• Empirical Formula (Hill Notation): C₂₃H₂₄N₆
• CAS Number: 1572414-83-5
• Molecular Weight: 384.48
• UNSPSC Code: 12352200
• NACRES: NA.77
• Assay: ≥98% (HPLC)
• Form: powder

Properties

• Quality Segment: 100
• assay: ≥98% (HPLC)
• form: powder
• color: white to beige
• solubility: DMSO: 20 mg/mL, clear
• storage temp.: 2-8°C
• SMILES string: [n]4(nncc4)c1ccc(cc1)CNc2nc(ncc2C)c3c(cccc3)C(C)C
• InChI: 1S/C23H24N6/c1-16(2)20-6-4-5-7-21(20)23-24-14-17(3)22(27-23)25-15-18-8-10-19(11-9-18)29-13-12-26-28-29/h4-14,16H,15H2,1-3H3,(H,24,25,27)
• InChI key: VUIRVWPJNKZOSS-UHFFFAOYSA-N

Description

Biochem/physiol Actions

ML323 is a potent selective inhibitor of the USP1–UAF1 deubiquitinase complex with an IC₅₀ of 76 nM in a ubiquitin-rhodamine (Ub-Rho) assay and excellent selectivity against other human deubiquitinases (DUBs), deSUMOylase, deneddylase and unrelated proteases. Human ubiquitin-specific protease 1 (USP1) associated with UAF1 is involved in the DNA damage response in the DNA translesion synthesis and Fanconi anemia pathways as the DUB responsible for deubiquitinating PCNA, which allows DNA replication past DNA lesions, and FANCD2 and FANCI, which function in interstrand crosslink repair. ML323 was found to potentiate cisplatin cytotoxicity in non-small cell lung cancer and osteosarcoma cells.

ML323 is a potent selective inhibitor of the USP1–UAF1 deubiquitinase complex.

ML323 induces viral replication in vitro and in vivo. It may act as a potential candidate for the intervention of diseases like rheumatoid arthritis and systemic lupus erythematosus.

Other Notes

ML323 has been expertly reviewed and recommended by the Chemical Probes Portal. For more information, please visit the ML323 probe summary on the Chemical Probes Portal website.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable