SA20

Starch (HK) Assay Kit

sufficient for 20 assays

• Synonym(s): Starch Quantitation Kit
• NACRES: NA.84
• UNSPSC Code: 12164500
• Technique(s): photometry: suitable
• Usage: sufficient for 20 assays
• Storage temp.: 2-8°C

Properties

• usage: sufficient for 20 assays
• Quality Segment: 100
• analyte chemical class(es): sugars
• technique(s): photometry: suitable
• application(s): food and beverages; general analytical
• storage temp.: 2-8°C

Description

Application

This kit is for the quantitative, enzymatic determination of native starch in food and other materials using the amyloglucosidase-hexokinase-glucose 6-phosphate dehydrogenase enzymatic assay. The hydrolysis of starch to glucose is catalyzed by amyloglucosidase. Glucose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The increase in absorbance at 340 nm is directly proportional to the glucose concentration.

Kit Components Only

• glucose assay reagent 20 mL
• starch assay reagent 1 mL/vial
• starch assay standard 5 g

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3