The presence of complement components and the activation cascade have not been investigated for this product. This is not included in the quality testing. However, the serum has not been heat inactivated. In theory, serum complement should still be present and the cascade system can be activated. The end user would have to determine the suitability of this product for compliment studies.
S5393
Serum minus IgA/IgM/IgG human
lyophilized powder
Synonym(s):
Depleted Serum
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About This Item
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
unconjugated
Species reactivity:
-
Application:
—
Citations:
17
Pricing and availability is not currently available.
biological source
human
Quality Segment
conjugate
unconjugated
form
lyophilized powder
contains
15 mM sodium azide
packaging
vial of 0.5 mL
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Serum is the liquid component of blood that includes proteins, immunoglobulin and electrolytes. The product can be used for selective removal of contaminating antibodies when immobilized on a solid matrix. This product can also be used in anti-protective antigen (anti-PA) IgG measurements using PA coupled microspheres[1]. Human serum deficient of IgG, IgA, and IgM can react specifically with anti-human whole serum but does not show reactivity with anti-human IgG, IgA, or IgM.
Application
Serum minus IgA/IgM/IgG human has been used as a control serum in immunoelectrophoresis, immunodiffusion,enzyme linked immunosorbent assay. It has also been used as
- control sera to assess neuraminidase inhibition
- microneutralization assay
- single radial hemolysis (SRH) repeatability assay
Preparation Note
Each vial contains at least 25 mg protein
Reconstitute with 0.5 mL deionized water.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| biological source human | biological source human | biological source - | biological source - |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| form lyophilized powder | form lyophilized powder | form buffered aqueous solution | form essentially salt-free, lyophilized powder |
| packaging vial of 0.5 mL | packaging vial of 0.5 mL | packaging - | packaging - |
| contains 15 mM sodium azide | contains 15 mM sodium azide | contains - | contains - |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Are the serum proteins of human complement cascade still active in the product S5393?
1 answer-
Helpful?
-
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How is the serum treated to deplete it from IgG, IgA and IgM?
1 answer-
Methods of preparation are considered proprietary.
Helpful?
-