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S2640

Serum Replacement 3 (50×)

liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Serum Substitute

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About This Item

NACRES:
NA.71
UNSPSC Code:
12352207
Sterility:
sterile-filtered
Form:
liquid
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sterility

sterile-filtered

Quality Segment

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

General description

Sigma′s Serum Replacement 3 is a fortified, multipurpose serum replacement for the long-term culturing of anchorage dependant and suspension cultures. This serum replacement is effective in research situations such as: studying the effects of growth factors or in the manufacturing of cell derived products.

Application

Serum Replacement 3 (50×) has been used as a supplement in modified phosphate-buffered saline (DPBS) and as a replacement for the growth medium for in vitro experiments for Tendon cells.

Analysis Note

Stable for 30 days when diluted in cell culture medium.

Other Notes

Defined serum replacement designed primarily for the long-term growth of human cells or other mammalian cells. Recommended for use in the production of cell-secreted proteins. Contains only human proteins (i.e., human serum albumin, human transferrin, human recombinant insulin). Does not contain growth factors, steroid hormones, glucocorticoids, cell adhesion factors, detectable Ig and mitogens. May be used with anchorage-dependent and suspension cells.

Disclaimer

This product is tested and found non-reactive for Hepatitis B Surface Antigen (HBsAg) and non-reactive for Human Immunodeficiency Virus (HIV) antibody by ELISA. Nevertheless, products of human origin should be considered potentially infectious and handled accordingly.

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Serum Replacement 3 (50×) liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

S2640

Serum Replacement 3 (50×)

Serum Replacement 2 (50×) liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

S9388

Serum Replacement 2 (50×)

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Sigma-Aldrich

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Sigma-Aldrich

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description

liquid, sterile-filtered, suitable for cell culture

description

liquid, sterile-filtered, suitable for cell culture

description

10 ×

description

-

form

liquid

form

liquid

form

powder

form

solution

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

sterility

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

titration: suitable

technique(s)

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

2-8°C

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

impurities

-

Quality Level

200

Quality Level

-

Quality Level

500

Quality Level

-

Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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Questions

1–2 of 2 Questions  

  1. What is the difference between Serum Replacement 1, 2, and 3?

    1 answer

    1. These concentrated serum replacements are defined, as multipurpose products designed to eliminate the need for serum in culture. These products do not contain growth factors, steroid hormones, or mitogens.

      S0638 Serum Replacement 1, 50X
      Total protein concentration at 1X of 770 mg/mL
      Highly purified bovine serum albumin (heat treated)
      Bovine transferrin (heat treated)
      Bovine insulin

      S9388 Serum Replacement 2, 50X
      Total protein concentration at 1X of 570 mg/mL
      Highly purified bovine serum albumin (heat treated)
      Bovine transferrin (heat treated)
      Bovine insulin

      S2640 Serum Replacement 3, 50X
      Total protein concentration at 1X of 664 mg/mL
      Highly purified human serum albumin (heat treated)
      Human transferrin (heat treated)
      Human (recombinant) insulin

      Helpful?

  2. Does the Serum Replacement 3 contain heat-treated bovine serum albumin, transferrin and insulin, like with Serum Replacement 1 and 2?

    1 answer

    1. As described in the "Other Notes" section of the product detail page, this is a defined serum replacement designed primarily for the long-term growth of human cells or other mammalian cells. Recommended for use in the production of cell-secreted proteins. Contains only human proteins (i.e., human serum albumin, human transferrin, human recombinant insulin). Does not contain growth factors, steroid hormones, glucocorticoids, cell adhesion factors, detectable Ig and mitogens. May be used with anchorage-dependent and suspension cells. It does not contain bovine proteins like products S0638, Serum Replacement 1 (50×) and S9388, Serum Replacement 2 (50×).

      Helpful?

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