The concentration of this product varies from lot to lot and may range from 20 - 40 mg/mL. At the minimum concentration of 20 mg/mL, the 10 mg package size would have a volume of 0.5 mL. This information is published in the product Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/r4642#product-documentation
R4642
Ribonuclease A from bovine pancreas
(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
Synonym(s):
Pancreatic Ribonuclease, RNAsea, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase
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About This Item
CAS Number:
NACRES:
NA.53
UNSPSC Code:
12352204
MDL number:
Biological source:
bovine pancreas
Concentration:
20-40 mg/mL
Pricing and availability is not currently available.
biological source
bovine pancreas
Quality Segment
grade
Molecular Biology
form
(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
mol wt
13.7 kDa, ~13,700
concentration
20-40 mg/mL
suitability
suitable for
foreign activity
Endonuclease and exonuclease, none detected, NICKase and DNase, none detected
storage temp.
−20°C
SMILES string
[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
General description
RNase A is an endoribonuclease that attacks at the 3′OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA.
RNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA.
Application
- RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.
- RNase A is also used in RNA sequence analysis and protection assays.
- RNase A has been used as a tool for computer-aided drug design.
- RNase A supports the analysis of RNA sequences.
- RNase A hydrolyze RNA contained in protein samples.
- Purification of DNA is supported by RNase A.
Suitable for:
- RNase protection assays
- Removal of unspecifically bound RNA
- Analysis of RNA sequences
- Hydrolysis of RNA contained in protein samples
- Plasmid DNA purification
Features and Benefits
Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification.
Analysis Note
Protein determined by E.
Other Notes
A major application for RNase A is the removal of RNA from preparations of plasmid DNA. For this application, DNase free RNase A is used at a final concentration of 10 ug/mL.
Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.
Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.
Activators of RNase A include potassium and sodium salts. RNase A can be inhibited by alkylation of His12 or His119.
RNase A is supplied as a solution of 50% glycerol containing 10 mM Tris-HCl (pH 8.0).
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This Item | |||
|---|---|---|---|
| biological source bovine pancreas | biological source - | biological source bovine pancreas | biological source bovine pancreas |
| grade Molecular Biology | grade Molecular Biology | grade - | grade - |
| form (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0) | form lyophilized | form solid | form lyophilized powder |
| concentration 20-40 mg/mL | concentration - | concentration ≥60% (SDS-PAGE) | concentration - |
| suitability suitable for | suitability - | suitability suitable for molecular biology | suitability suitable for mRNA or total RNA extracted from cells and tissues |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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Danger
hcodes
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Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the Total Volume of this product?
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What is the actual concentration of 10MG R4642, Ribonuclease A in terms of mg/ml?
1 answer-
The mg/mL concentration of this product is lot dependent and reported in the product Certificate of Analysis. The specification for the concentration is 20 - 40 mg/mL. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/r4642#product-documentationPlease see the link below to review additional information is the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/316/984/r4642dat.pdfHelpful?
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When using Product R4642, Ribonuclease A from bovine pancreas (RNase A), do I need to boil the RNase A to inactivate residual DNases?
1 answer-
Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity.
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Why is RNase A activity not listed as micromoles per minute, and what is a Kunitz unit?
1 answer-
The Kunitz unit was created to reflect the non-linear nature of the RNAse enzymatic reaction. As the reaction progresses the activity changes due to RNA as a heterogeneous polymeric substrate; therefore, the unit of moles or micomoles per minute becomes inaccurate. As such, our enzymatic assay describes a unit that varies throughout the reaction: One unit will cause a decrease of 100% per minute in the value of E0 - Ef at pH 5.0 at 25°C. Ef is determined in the RNAse control step, while E0 reflects the current reaction rate.
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Product R4642, Ribonuclease A from bovine pancreas (RNase A), is a solution. What does the solution contain?
1 answer-
The product is supplied in a solution containing 50% glycerol and 10 mM Tris-HCl, pH 8.0.
Helpful?
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What is the shelf life of Product R4642, Ribonuclease A from bovine pancreas (RNase A)?
1 answer-
This product is stable for at least 2 years when stored properly at -20 °C.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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