No, this product does not contain a His-tag. See the link below for additional product information: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/394/024/p7582dat.pdf
P7582
Amino-terminal FLAG-BAP™ Fusion Protein
Synonym(s):
Amino-terminal Fusion Protein, BAP Fusion Protein, FLAG-BAP Fusion Protein
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About This Item
Form:
liquid
Mol wt:
~49 kDa
Pricing and availability is not currently available.
form
liquid
Quality Segment
mol wt
~49 kDa
technique(s)
FLAG purification: suitable
shipped in
dry ice
storage temp.
−20°C
General description
Amino-terminal FLAG-BAP™ Fusion Protein is a 467 amino acid N-terminal FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular mass of 49.3kDa. The N-terminal FLAG-BAP Fusion Protein migrates as a 45−55kDa band by SDS-PAGE depending on electrophoresis conditions. FLAG-BAP™ Fusion Protein has biotechnological applications and is used in the screening of recombinant fusion proteins.
The Amino-terminal FLAG-BAP Fusion Protein is a control prorein used to confirm the funtional integrity of the anti-FLAG M1 and M2 monoclonal antibodies in applications such as western blotting, immunoprecipitation, ELISA, electron microscopy, and FACS.
Application
Amino-terminal FLAG-BAP™ Fusion Protein has been used as a standard in enzyme-linked immunosorbent assay (ELISA) for osteopontin quantification and in western blotting based quantification of Flag-Wnt8 and Drosophila melanogaster transcription factor interactome.
Learn more product details in our FLAG® application portal.
Physical form
Protein is supplied in 10 mM Tris, 120 mM NaCl,
0.05 mM ZnCl2 in 50% glycerol, pH 8.0.
0.05 mM ZnCl2 in 50% glycerol, pH 8.0.
Other Notes
Control protein
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
|---|---|---|---|
| description - | description - | description - | description lyophilized powder |
| form liquid | form liquid | form - | form lyophilized powder |
| mol wt ~49 kDa | mol wt ~49 kDa | mol wt ~49 kDa | mol wt 1012.97 Da |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in wet ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Storage Class
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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