P4798

L-Phenylalanine Dehydrogenase from Sporosarcina sp.

lyophilized powder, ≥6 units/mg solid

• CAS Number: 69403-12-9
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 1.4.1.20 (BRENDA, IUBMB)
• MDL number: MFCD00151723
• Specific activity: ≥6 units/mg solid
• Biological source: bacterial (Sporosarcina sp.)
• Concentration: ≤100%

Properties

• biological source: bacterial (Sporosarcina sp.)
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥6 units/mg solid
• storage condition: dry at room temperature
• concentration: ≤100%
• color: white to light brown
• application(s): life science and biopharma
• storage temp.: −20°C

Description

General description

Research area: CELL SIGNALING

Phenylalanine dehydrogenase is a member of a large family of amino-acid dehydrogenases, which includes glutamate dehydrogenase, alanine dehydrogenase, leucine dehydrogenase, lysine ε-dehydrogenase, and meso-a,ε-diaminopimelate D-dehydrogenase. The three known gene sequences are octomers. It has a two-domain, three-dimensional structure.

Biochem/physiol Actions

Phenylalanine dehydrogenase (PheDH) is considered an effective enzyme to estimate the quantity of phenylalanine to distinguish phenylketonuria (PKU) disease.Moreover, it is utilized for the production of optically pure l-phenylalanine, a key component of the artificial sweetener aspartame. L-Phenylalanine dehydrogenase is a NAD+-dependent oxidoreductase that catalyzes the reversible, oxidative deamination of L-phenylalanine, which results in its degradation. L-Phenylalanine dehydrogenase is used to study phenylalanine metabolism and phenylalanine, tyrosine, and tryptophan biosynthesis.

Other Notes

One unit will oxidize 1.0 μmole of L-phenylalanine per min at pH 10.5 at 30 °C in the presence of β-NAD.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)