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Protease from Aspergillus saitoi

Name: Protease from <I>Aspergillus saitoi</I>
Brand: SIGMA

Type XIII, ≥0.6 unit/mg solid

Synonym(s):

Molsin

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About This Item

CAS Number:

9025-49-4

EC Number:

3.4.23.18 (BRENDA, IUBMB)

UNSPSC Code:

12352204

EC Number:

232-796-2

NACRES:

NA.54

eCl@ss:

32160410

MDL number:

MFCD00132092

Specific activity:

≥0.6 unit/mg solid

Biological source:

Aspergillus sp. (Aspergillus saitoi)

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Properties

biological source

Aspergillus sp. (Aspergillus saitoi)

Quality Segment

200

type

Type XIII

form

solid

specific activity

≥0.6 unit/mg solid

foreign activity

alkaline protease, essentially free

storage temp.

−20°C

Description

Application

Protease from Aspergillus saitoi has been used in a study to assess sequence coverage and resolution in hydrogen exchange of large proteins. [1] It has also been used in a study to investigate the conversion of soybean curd isoflavone glycosides to their aglycones through β-glucosidase.. [2]

Biochem/physiol Actions

Protease from Aspergillus saitoi was shown to also function as a β-glucosidase. [2]

Other Notes

One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 2.8 at 37 °C (color by Folin-Ciocalteu reagent).

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This Item	P4032	62301	86247
Sigma-Aldrich P2143 Protease from Aspergillus saitoi	Sigma-Aldrich P4032 Proteinase from Aspergillus melleus	Sigma-Aldrich 62301 Lipase from Aspergillus niger	Sigma-Aldrich 86247 Taka-Diastase from Aspergillus oryzae
biological source Aspergillus sp. (Aspergillus saitoi)	biological source Aspergillus sp. (A. melleus)	biological source Aspergillus niger	biological source Aspergillus sp. (A. oryzae)
specific activity ≥0.6 unit/mg solid	specific activity ≥3 units/mg solid	specific activity ~200 U/g	specific activity ~100 U/mg
form solid	form solid	form powder (fine)	form powder
storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
foreign activity alkaline protease, essentially free	foreign activity -	foreign activity -	foreign activity -
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 200