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P2124

Sigma-Aldrich

Anti-phospho-Polo-Like Kinase Kinase (pSer482, pSer486, pSer490) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Anti-phospho-PLKK

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About This Item

MDL number:
MFCD08705499
UNSPSC Code:
12352203
NACRES:
NA.44

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

mol wt

antigen ~120 kDa

species reactivity

Xenopus

technique(s)

western blot: 1:1,000 using Xenopus lysates

shipped in

wet ice

storage temp.

−20°C

Gene Information

Xenopus laevis ... stk10-a(373731)

General description

Polo-Like Kinase Kinase (PLKK) is a 120kD protein that belongs to the serine/threonine kinase family. It has a crucial role in regulating cellular events in cytokinesis and mitosis. Anti-phospho-polo-like kinase kinase (pSer482, pSer486, pSer490) antibody can be used in immunoblotting (diluted 1:1000). Rabbit anti- phospho-polo-like kinase kinase antibody reacts specifically with phospho-polo-like kinase kinase of xenopus.

Immunogen

synthetic phosphopeptide corresponding to amino acids residues surrounding the Xenopus polo-like kinase kinase phosphorylated on serines 482, 486 and 490.

Application

Anti-phospho-polo-like kinase kinase (pSer482, pSer486, pSer490) antibody can be used in western blotting (diluted 1:1000).

Physical form

Solution in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Xingjie Ma et al.
Aging cell, 17(6), e12831-e12831 (2018-09-15)
Calcium signaling is emerging as a key pathway controlling cellular senescence, a stable cell proliferation arrest playing a fundamental role in pathophysiological conditions, such as embryonic development, wound healing, cancer, and aging. However, how calcium signaling is regulated is still
Y W Qian et al.
Science (New York, N.Y.), 282(5394), 1701-1704 (1998-11-30)
The Xenopus polo-like kinase 1 (Plx1) is essential during mitosis for the activation of Cdc25C, for spindle assembly, and for cyclin B degradation. Polo-like kinases from various organisms are activated by phosphorylation by an unidentified protein kinase. A protein kinase

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