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N6539

Sigma-Aldrich

Anti-NUMB (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-S171

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

mol wt

antigen ~70 kDa

species reactivity

human, rat

packaging

antibody small pack of 25 μL

concentration

~1.0 mg/mL

technique(s)

western blot: 1-2 μg/mL using rat brain extract (S1 fraction) and A431 cell lysate

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NUMB(8650)
mouse ... Numb(18222)
rat ... Numb(29419)

General description

Numb gene with 13 exons is mapped to human chromosome 14q24.3. The protein comprises 651 amino acids. It belongs to the clathrin-associated sorting protein family with two domains namely phosphotyrosine-binding (PTB) and proline-rich region (PRR) domain. Mammalian Numb gene gives rise to at least four alternatively spliced transcripts that produce four protein isoforms Numb1-4, ranging from 65 to 72 kDa.
Numb homolog-NUMB is an adaptor protein and is aberrantly expressed in cancers.

Application

Anti-NUMB (C-terminal) antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Numb homolog-NUMB may act as a tumor suppressor. It also takes part in determining the cell fate. NUMB is involved in the endocytosis and trafficking of receptors.
Numb is a critical Notch antagonist and a conserved adapter protein that plays a role in the self-renewal of NSCs and neural differentiation in the central nervous system (CNS). In neural progenitors of CNS, the functional loss of numb is implicated in embryonic lethality accompanied by precocious neuronal differentiation. It has been suggested that a switch in numb isoform expression is a critical step in cortical development. Numb1 or numb3 isoforms that contain an insertion in the proline-rich region (PRRL), promote the proliferation of neural crest stem cells (NCSCs). Numb2 or numb4 isoforms without PRR insertions (PRRS) show ectopic expression and promote neuronal differentiation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Distinct functions of human numb isoforms revealed by misexpression in the neural stem cell lineage in the Drosophila larval brain
Toriya M, et al.
Developmental Neuroscience, 28(1-2), 142-142 (2006)
A switch in numb isoforms is a critical step in cortical development
Bani-Yaghoub M, et al.
Developmental Dynamics, 236(3), 696-705 (2007)
Yinying Lu et al.
Hepatology (Baltimore, Md.), 62(4), 1122-1131 (2015-06-11)
The cell fate determinant Numb is aberrantly expressed in cancer. Numb is alternatively spliced, with one isoform containing a long proline-rich region (PRR(L) ) compared to the other with a short PRR (PRR(S) ). Recently, PRR(L) was reported to enhance
Notch: from neural development to neurological disorders
Lathia JD, et al.
Journal of Neurochemistry, 107(6), 1471-1481 (2008)
Phenotypic and genetic characterization of a patient with a de novo interstitial 14q24. 1q24. 3 deletion
Tassano E, et al.
Molecular Cytogenetics, 7(1), 49-49 (2014)

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