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Nile Blue A

Name: Nile Blue A
Brand: SIGMA

Dye content ≥75 %, Powder

Synonym(s):

Basic Blue 12, Nile blue sulfate

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About This Item

Linear Formula:

2C₂₀H₂₀N₃O · SO₄

CAS Number:

3625-57-8

Molecular Weight:

732.85

NACRES:

NA.47

PubChem Substance ID:

24897743

UNSPSC Code:

12171500

EC Number:

222-832-5

MDL number:

MFCD00064529

Colour Index Number:

51180

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Properties

Product Name

Nile Blue A, Dye content ≥75 %

form

powder

Quality Segment

100

composition

Dye content, ≥75%

mp

>300 °C (dec.) (lit.)

solubility

H₂O: 1 mg/mL, blue

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[O-]S([O-])(=O)=O.CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)cc(N)c4ccccc34.CC\[N+](CC)=C5/C=CC6=Nc7c(OC6=C5)cc(N)c8ccccc78

InChI

1S/2C20H19N3O.H2O4S/c2*1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;1-5(2,3)4/h2*5-12,21H,3-4H2,1-2H3;(H2,1,2,3,4)

InChI key

QIRDPEPUXNCOLD-UHFFFAOYSA-N

Description

General description

Nile Blue A is aminodiethylaminonaphthophenoxazonium sulfate employed in biology and histology. It is a highly fluorescent, and photostable organic dye used to dye clothing, and is for use in lipid histochemistry.

Application

Nile blue A is suitable for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue, and in a histochemical method for lipids in frozen sections.

Biochem/physiol Actions

Nile blue A stains acidic components, such as phospholipids, nucleic and fatty acids by imparting a dark blue color, while neutral lipids, namely triglycerides, are stained pink, or red.

Features and Benefits

Used in the preparation of an amperometric glucose sensor.

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This Item	N0766	N3013	391557
Sigma-Aldrich N5632 Nile Blue A	Sigma-Aldrich N0766 Nile Blue A	Sigma-Aldrich N3013 Nile Red	Sigma-Aldrich 391557 Oil Blue N
application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology
form powder	form powder	form powder	form powder
solubility H₂O: 1 mg/mL, blue	solubility H₂O: 1 mg/mL	solubility methanol: 1 mg/mL	solubility acetone: 1%, turbid, blue to very deep blue
storage temp. room temp	storage temp. room temp	storage temp. room temp	storage temp. room temp
composition Dye content, ≥75%	composition Dye content, ≥75%	composition Carbon: 70.0-77.3% Nitrogen: 7.5-8.8%	composition Dye content, 96%
Quality Level 100	Quality Level 100	Quality Level 200	Quality Level 200

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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