This product is soluble in water at 50 mg/mL. For cell culture use, ultrapure water is typically preferred. Dimethyl sulfoxide (DMSO) is optional and should be used only when an organic solvent is required, with solubility up to approximately 100 mM. Please see the link below to access a sample or lot-specific Certificate of Analysis: https://www.sigmaaldrich.com/product/sigma/n0636#product-documentation
N0636
Nicotinamide
BioReagent, suitable for cell culture, suitable for insect cell culture
Synonym(s):
Niacinamide, Nicotinic acid amide, Pyridine-3-carboxylic acid amide, Vitamin B3, Vitamin PP
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About This Item
Empirical Formula (Hill Notation):
C6H6N2O
CAS Number:
Molecular Weight:
122.12
UNSPSC Code:
12352205
NACRES:
NA.75
PubChem Substance ID:
EC Number:
202-713-4
Beilstein/REAXYS Number:
383619
MDL number:
Pricing and availability is not currently available.
biological source
synthetic (organic)
Quality Segment
product line
BioReagent
assay
≥98% (HPLC)
form
powder
mol wt
Mw 122.12 g/mol
technique(s)
cell culture | insect: suitable, cell culture | mammalian: suitable
color
white
mp
128-131 °C (lit.)
solubility
water: soluble 50 mg/mL, clear, colorless
SMILES string
NC(=O)c1cccnc1
InChI
1S/C6H6N2O/c7-6(9)5-2-1-3-8-4-5/h1-4H,(H2,7,9)
InChI key
DFPAKSUCGFBDDF-UHFFFAOYSA-N
Gene Information
human ... PARP1(142), SIRT2(22933)
General description
Nicotinamide (NAM) belongs to vitamin B family and is the amide form of vitamin B3. NAM is present in food and is a constituent of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP). Tryptophan is the precursor for NAM synthesis in liver. NAM controls cell metabolism, redox reactions, mitochondrial functionsand energy generation. It promotes natural killer cells proliferation and survival. NAM confers neuroprotection and promotes neuronal maturation. It has the ability to cross the blood-brain barrier and may provide protection in ischaemic stroke and traumatic brain injury. NAM may elicit therapeutic benefits in Alzheimer′s, Parkinson′s, and Huntington′s diseases.
Application
Nicotinamide has been used as a medium supplement in organoid culture of intestinal stem cells, esophageal squamous cell carcinomas(ESCC), pancreatic cancer organoids and pancreatic progenitor induction.
Biochem/physiol Actions
Nicotinamide is an amide derivative of vitamin B3 and a PARP inhibitor
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This Item | |||
|---|---|---|---|
| technique(s) cell culture | insect: suitable, cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) gas chromatography (GC): suitable, liquid chromatography (LC): suitable |
| biological source synthetic (organic) | biological source synthetic | biological source - | biological source - |
| assay ≥98% (HPLC) | assay ≥99.5% (HPLC) | assay ≥98% (HPLC) | assay - |
| form powder | form powder or crystals | form powder | form solid |
| solubility water: soluble 50 mg/mL, clear, colorless | solubility - | solubility - | solubility - |
| color white | color colorless to white | color white | color - |
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signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
302.0 °F - closed cup
flash_point_c
150 °C - closed cup
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Is it best to use ultrapure water or DMSO as a solvent for making stock solution? I plan to use it for cell culture.
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what is the solubility in DMSO?
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Nicotinamide is soluble in DMSO to 100 mM.
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What is the stability of Nicotinamide when lyophilized and in stock solution?
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The recommended storage conditions and stability for stock solutions, once prepared, are stored aliquoted in tightly sealed vials at -20°C or below and used within 1 month. Wherever possible, solutions should be made and used on the same day.
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Can Nicotinamide aliquots be freeze-thawed more than once?
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The product in solution has not been tested for stability following multiple freeze/thaw cycles. As a general practice, multiple freeze/thaw cycles are avoided.
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How long are aliquots of a 1 M solution in DPBS or water at °70°C stable?
1 answer-
Solution stability studies at 70°C have not been performed on this product. Neutral aqueous solutions are fairly stable and may be autoclaved at 120 °C for up to 20 minutes. Solutions at an alkaline pH will be hydrolyzed to nicotinic acid. Nicotinamide is a component of several vitamin solutions used in cell culture (listed as niacinamide). These products are stored at -20°C, but are typically added to culture medium at 37°C.
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Which is the recomiendes re test date for N0636-100g, lot SLBZ7561? Thank you.
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This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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