The concentration of the p-tyramine included in the kit is 100mM, and the enzyme concentration is 0.02 U/uL.
MAK136
Monoamine Oxidase Activity Assay Kit
Monoamine Oxidase Activity Assay Kit
sufficient for 100 fluorometric tests
Synonym(s):
MAO Assay Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.
Recommended Products
usage
sufficient for 100 fluorometric tests
detection method
fluorometric
relevant disease(s)
neurological disorders
storage temp.
−20°C
Gene Information
human ... MAOA(4128) , MAOB(4129)
mouse ... MAOA(17161) , MAOB(109731)
rat ... MAOA(29253) , MAOB(25750)
General description
Monoamine oxidases (MAO, EC 1.4.3.4) are a family of mitochondrial enzymes that catalyze the oxidative deamination of monoamines. In mammals, MAO consists of two isoenzymes, MAO-A and MAO-B. MAO dysfunction is thought to be responsible for a number of neurological disorders. Unusually high or low levels of MAO in the body have been associated with depression, schizophrenia, substance abuse, attention deficit disorder, migraines, and irregular sexual maturation. MAO inhibitors are one of the major classes of drug prescribed for the treatment of depression.
Application
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the determination of MAO activity in biological samples and for the screening of MAO inhibitors. Can determine either MAO-A, MAO-B or both MAO-A and MAO-B activity.
Principle
The Monoamine Oxidase Assay Kit provides a convenient fluorimetric means to measure MAO enzyme activity in biological samples. In the assay, MAO reacts with p-tyramine, a substrate for both MAO-A and MAO-B, resulting in the formation of H2O2, which is determined by a fluorimetric method (λex = 530/λem = 585 nm). The assay is simple, sensitive, stable, and high-throughput adaptable. Unit definition: one unit of MAO catalyzes the formation of 1 μmole of H2O2 per minute under the assay conditions.
Storage Class Code
10 - Combustible liquids
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What is the concentration of the p-tyramine stock and the HRP enzyme that is included in the kit?
1 answer-
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Is it possible to determine if this kit would be suitable for the quantitation of MAO-N?
1 answer-
This kit is not tested with MAO-N. It is important to note that this kit was specifically developed with MAO-A and MAO-B in mind, and it is uncertain whether other enzymes, such as MAO-N, would be compatible with the substrate.
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