The mannan used in the product of this product is from Saccharomyces cerevisiae. As stated in the 'PROPERTIES' section, the matrix attachment is at the hydroxyl group. The minimum binding capacity is 30 mg/mL using Concanavalin A.
M9917
Mannan−Agarose
saline suspension
Synonym(s):
Mannan Binding Protein Agarose
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About This Item
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biological source
plant
Quality Level
form
saline suspension
technique(s)
affinity chromatography: suitable
matrix
cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
matrix attachment
hydroxyl
matrix spacer
1 atom
capacity
≥30 mg/mL binding capacity (concanavalin A)
suitability
suitable for chromatography
storage temp.
2-8°C
Application
Mannan-agarose is used in protein chromatography, affinity chromatography[1] and carbohydrate matrices. Mannan-agarose has been used in studies assessing poly(ADP-ribosyl)ation of mannose-binding lectin out of human kidney cells and to inform mannose-associated serine protease (MASP) activated components of the coagulation system. Mannan-agarose has also been used to study the innate immunity and resistance to Aeromonas salmonicida exhibited by the sea lamprey (Petromyzon marinus), a parasitic cartilaginous fish of the North American Great Lakes and a predator of many bony fish species of commercial importance to the fishing industry. Mannan-Agarose has been used to deplete rabbit anti-mannose-binding lectin (MBL) polyclonal antiserum of anti-mannan antibodies.[2]
Physical form
Suspension in 1.0 M NaCl, 10 mM imidazole containing preservative
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Complement plays a significant role in mediating endothelial injury following oxidative stress. We have previously demonstrated that the lectin complement pathway (LCP), which is initiated by deposition of the mannose-binding lectin (MBL), is largely responsible for activating complement on endothelial
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Can you please provide details of the #m9917 mannan-agarose that you supply. E.g. the origin of the mannan immobilized (yeast?) and how it was immobilized (the data in the website referes to CNBr but I wonder how this works with a sugar. Thank you.
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