Key Documents

View All Documentation

Minimum Essential Medium Eagle

Name: Minimum Essential Medium Eagle
Brand: SIGMA

With Hanks′ salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

EMEM, MEM

Select a Size

Change View

Size/SKU	Availability	Price

About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

Pricing and availability is not currently available.

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Product Name

Minimum Essential Medium Eagle, With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

glucose: yes
Hanks’ salts (2% CO₂): yes
L-glutamine: yes
Earle’s salts (5% CO₂): no
NaHCO₃: no
phenol red: yes
sodium pyruvate: no
stable glutamine: no
HEPES: no

shipped in

ambient

storage temp.

2-8°C

Description

General description

Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has high concentrations of amino acids, so the medium closely approximates the protein concentration of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has increased the applicability of this medium. This medium lacks calcium that permits the growth of cells in suspension. Modification of the original MEM formulation. Earle′s salts have been replaced with Hanks′ salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity.

Application

Minimum Essential Medium Eagle (MEM) has been used:

as a component of the Hank′s MEM (HMEM)-Pipes buffer to induce anaphylatoxin induced-ear swelling in mice
as a component of the slice culture medium (SCM) for the incubation of mouse hippocampal slice cultures
to culture Müller cells from guinea pigs

Preparation Note

Formulated to contain 10.6 grams of powder per liter of medium.

Supplement with 0.35 g/L sodium bicarbonate.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	M0644	M4526	M5650
Sigma-Aldrich M4642 Minimum Essential Medium Eagle	Sigma-Aldrich M0644 Minimum Essential Medium Eagle	Sigma-Aldrich M4526 Minimum Essential Medium Eagle	Sigma-Aldrich M5650 Minimum Essential Medium Eagle
description With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture	description Alpha Modification, with L-glutamine, ribonucleosides and deoxyribonucleosides, without sodium bicarbonate, powder, suitable for cell culture	description Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture	description With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
form powder	form powder	form liquid	form liquid
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
components glucose: yes Hanks’ salts (2% CO₂): yes L-glutamine: yes Earle’s salts (5% CO₂): no NaHCO₃: no phenol red: yes sodium pyruvate: no stable glutamine: no HEPES: no	components NaHCO₃: no L-glutamine: yes phenol red: yes HEPES: no sodium pyruvate: yes	components Earle’s salts (5% CO₂): yes sodium pyruvate: yes glucose: yes L-glutamine: no HEPES: no Hanks’ salts (2% CO₂): no phenol red: yes NaHCO₃: yes stable glutamine: no	components phenol red: yes NaHCO₃: yes L-glutamine: no sodium pyruvate: no HEPES: no Earle’s salts (5% CO₂): yes
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
Minimum Essential Medium Eagle for lab	classic media, classical media, FBS, hybrid cells	classic media, classical media, MEM-alpha, classic media, classical media, FBS	classic media, classical media, MEM, classic media, classical media, FBS

Still not finding the right product?

Explore all of our products under Minimum Essential Medium Eagle

Storage Class

11 - Combustible Solids

WGK

WGK 1

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.