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Minimum Essential Medium Eagle

Name: Minimum Essential Medium Eagle
Brand: SIGMA

HEPES modification, with Earle′s salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

EMEM, MEM

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

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Properties

Product Name

Minimum Essential Medium Eagle, HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: 25 mM
phenol red: yes
L-glutamine: yes
NaHCO₃: no
sodium pyruvate: no
Earle’s salts (5% CO₂): yes

shipped in

ambient

storage temp.

2-8°C

Description

Preparation Note

Formulated to contain 14.2 grams of powder per liter of medium.

Supplement with 2.2 g/L sodium bicarbonate.

Other Notes

Leaving the bicarbonate out gives the investigator greater control over the buffer system.

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This Item	M0518	M0275	M8167
SAFC M2645 Minimum Essential Medium Eagle	Sigma-Aldrich M0518 Minimum Essential Medium Eagle	Sigma-Aldrich M0275 Minimum Essential Medium Eagle	Sigma-Aldrich M8167 Minimum Essential Medium Eagle
description HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture	description Joklik Modification, with L-glutamine, without calcium chloride and sodium bicarbonate, suitable for cell culture	description 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture	description Spinner Modification, with Earle′s salts and sodium bicarbonate, without calcium chloride and L-glutamine, liquid, sterile-filtered, suitable for cell culture
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form powder	form powder	form liquid	form liquid
components HEPES: 25 mM phenol red: yes L-glutamine: yes NaHCO₃: no sodium pyruvate: no Earle’s salts (5% CO₂): yes	components HEPES: no sodium pyruvate: no L-glutamine: yes NaHCO₃: no phenol red: yes	components phenol red: yes HEPES: no Earle’s salts (5% CO₂): yes L-glutamine: no sodium pyruvate: no NaHCO₃: no	components sodium pyruvate: no phenol red: yes Earle’s salts (5% CO₂): yes NaHCO₃: yes L-glutamine: no HEPES: no
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
classic media, classical media, FBS	classic media, classical media, FBS, suspension cultures	MEM, classic media, classical media, FBS	MEM-Spinner, classic media, classical media, FBS

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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